Ready-to-use media
Biggy Agar
Culture medium for the isolation and identification of Candida sp.
- Cat. No.
- LC-920
- Category
- Ready-to-use media
- Format
- 20 plates, 90 mm
- Storage
- 2 to 14 °C
- Minimum guaranteed shelf life
- 1 month
- Also available
- Dehydrated medium, 1 format (LC-1006)
Nickerson Agar (BIGGY) is suitable for the isolation and identification of Candida species. The medium is made according to the general principles of Bismuth-Sulfite Agar. An inhibitory and differential medium using a high concentration of glycine for selectivity. This medium is highly inhibitory, and does not allow bacterial growth, however most Candida spp. grow freely and rapidly. Occasionally, tiny colonies of bacteria or highly repressed moulds may appear.
Key features
Applications
Selective isolation of Candida.
Industry
Clinical.
Presentation
20 prepared plates, 90 mm, with 21 ± 2 mL.
Packaging
1 box with 2 packs of 10 plates/pack. Single cellophane.
Description and technique
The appearance of the colonies in this medium after an incubation of 48-72 hours at 28-30 °C is as follows:
- Candida albicans: Creamy colonies, very convex, circular with very slight mycelial border and black or dark brown in colour. It has no metallic sheen or diffused pigment, even after 72 hours of incubation.
- Candida tropicalis: Acuminated colonies, creamy, irregular and with slight mycelial borders. Dark brown with black centre. After 72 hours of incubation the colonies may take on a metallic sheen and produce a diffused zone of pigment.
- Candida krusei: Big and plain colonies, with irregular borders. Brown colour, darker in the centre. A yellow halo appears around the colony.
- Candida parakrusei: Plain colonies, average size, irregular. Dark red centre and light red borders, but when the border is mycelial it looks yellow.
- Candida pseudotropicalis: Big and plain colonies, dark red colour with mycelial border.
- Candida stellatoidea: Average size plain colonies, dark brown colour, without mycelial development.
- Rhodotorula: Creamy convex colonies, with irregular border and colours ranging from pink to orange.
- Moulds in general: Restricted colonial growth and cottony appearance.
Note: A decrease in pH is normal and does not affect performance.
Presentation
| Presentation | 20 prepared plates, 90 mm |
|---|---|
| Filling | 21 ± 2 mL |
| Packaging | 1 box with 2 packs of 10 plates/pack. Single cellophane. |
Composition
| Ingredient | g/L |
|---|---|
| Yeast extract | 1.00 |
| Dextrose | 10.00 |
| Glycine | 10.00 |
| Sodium sulfite | 3.00 |
| Ammonium bismuth citrate | 5.00 |
| Agar | 15.00 |
Note: A decrease in pH is normal and does not affect performance.
Quality control
Physical/chemical control
| Color | Off-white |
|---|---|
| pH | 6.8 ± 0.2 at 25 °C |
Microbiological control
Spiral spreading: practical range 100 ± 20 CFU; min. 50 CFU (productivity) / 10⁴-10⁶ CFU (selectivity).
Aerobiosis. Incubation at 28 ± 2 °C during 24-72 h.
| Microorganism | Growth |
|---|---|
| Candida albicansATCC 10231, WDCM 00054 | Good. Dark brown colonies. |
| Candida tropicalisATCC 1369 | Good. Dark brown colonies. |
| Escherichia coliATCC 25922, WDCM 00013 | Inhibition (partial to complete) |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ATLAS, R.M. & L.C. PARKS (1993) Handbook of Microbiological Media. CRC Press. Boca Raton. Fla. USA.
- FORBES, B.A., D.F. SAHM & A.S. WEISSFELD (1998) Bailey & Scott's Diagnostic Microbiology. Mosby. St Louis. MO. USA.
- ISENBERG, H.D. (1995) Clinical Microbiology Procedures Handbook. ASM Press. Washington. DC. USA.
- MacFADDIN, J.D. (1985) Media for isolation-cultivation-identification-maintenance of medical bacteria. William & Wilkins. Baltimore. MD. USA.
- MURRAY, P.R., E.J. BARON, J.H. JORGENSEN, M.A. PFALLER & R.H. YOLKEN (Eds) (2003) Manual of Clinical Microbiology. 8th ed. ASM Press. Washington. DC. USA.
- NICKERSON (1953) Reduction of Inorganic substance by yeast I. Extracellular reduction of sulfite by species of Candida. J. Inf. Dis 93:43.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
Related products
Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.