Ready-to-use media
Brain Heart Infusion Agar (BHI Agar)
General purpose medium for isolation and culture of pathogenic microorganisms.
- Cat. No.
- LC-937
- Category
- Ready-to-use media
- Format
- 20 plates, 90 mm
- Storage
- 2 to 14 °C
- Minimum guaranteed shelf life
- 1 month
- Minimum order
- 15 packs
- Also available
- Dehydrated medium, 1 format (LC-1048)
Brain Heart Infusion is used for the cultivation of fastidious bacteria (streptococci, pneumococci, meningococci, etc.) and is also recommended for the cultivation of pathogenic fungi.
Key features
Applications
Non-selective enumeration of fastidious microorganisms.
Industry
Clinical.
Presentation
20 prepared plates, 90 mm, with 21 ± 2 mL.
Packaging
1 box with 2 packs of 10 plates/pack. Single cellophane.
Description and technique
Collect, dilute and prepare samples and volumes as required according to specifications, directives, official standard regulations and/or expected results.
Spread the plates by streaking methodology or by spiral method.
Incubate the plates right side up aerobically at 37 ± 1 °C for 24-48 h.
(Incubation times greater than those mentioned above or different incubation temperatures may be required depending on the sample, on the specifications, etc. This medium can be inoculated directly or after enrichment broth.)
After incubation, enumerate all the colonies that have appeared onto the surface of the agar.
Each laboratory must evaluate the results according to their specifications.
Calculate total microbial count per mL of sample by multiplying the average number of colonies per plate by the inverse dilution factor if a diluted sample was streaked. Report results as colony forming units (CFU) per mL or g along with incubation time and temperature.
Presentation
| Presentation | 20 prepared plates, 90 mm |
|---|---|
| Filling | 21 ± 2 mL |
| Packaging | 1 box with 2 packs of 10 plates/pack. Single cellophane. |
Composition
| Ingredient | g/L |
|---|---|
| Brain extract | 12.5 |
| Heart extract | 5.0 |
| Proteose peptone | 10.0 |
| Sodium chloride | 5.0 |
| Disodium phosphate | 2.5 |
| Dextrose | 2.0 |
| Agar | 15.0 |
Quality control
Physical/chemical control
| Color | Yellow |
|---|---|
| pH | 7.4 ± 0.2 at 25 °C |
Microbiological control
Inoculate: practical range 100 ± 20 CFU; min. 50 CFU (productivity).
Microbiological control according to ISO 11133:2014/Amd 1:2018.
Aerobiosis. Incubation at 37 ± 1 °C, reading after 24-48 ± 2 h.
| Microorganism | Growth |
|---|---|
| Stph. aureusATCC 25923, WDCM 00034 | Good (≥ 70%) |
| Staphylococcus aureusATCC 6538, WDCM 00032 | Good (≥ 70%) |
| Streptococcus pyogenesATCC 19615 | Good (≥ 70%) |
| Streptococcus pneumoniaeATCC 49619 | Good (≥ 70%) |
| Enterococcus faecalisATCC 19433, WDCM 00009 | Good (≥ 70%) |
| Escherichia coliATCC 8739, WDCM 00012 | Good (≥ 70%) |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- AJELLO, L., L.K. GEORG, W. KAPLAN & L. KAUFMAN (1966) Laboratory Manual for Medical Mycology. (CDC) US DHEW, Center for Disease Control. Atlanta.
- APHA-AWWA-AWPC (1998) Standard methods for the examination of water and wastewater. 20th ed. Washington. DC. USA.
- ATLAS, R.M. & L.C. PARKS (1993) Handbook of microbiological Culture Media. CRC Press. London.
- DOWNES, F.P. & K. ITO (2001) Compendium of methods for the microbiological examination of foods. APHA. Washington. DC. USA.
- FDA (Food and Drug Administration) (1998) Bacteriological Analytical Manual. 8th ed. Rev. A. AOAC International. Gaithersburg. VA. USA.
- HAYDEN, R.L. (1923) Elective localization in the eye of bacteria from infected teeth. Arch. Int. Med. 32:828-849.
- HOWELL, A. (1948) The efficiency of methods for the isolation of Histoplasma capsulatum. Public Health Reports, 63:173-178.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- ROSENOW, E.C. (1919) Studies on elective localization. Focal infection with special reference to oral sepsis. J. Dental Res. 1:205-249.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.