Dehydrated culture media
Dextrose Agar
For the cultivation of a wide variety of microorganisms, with or without blood added.
- Cat. No.
- LC-1021
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Dextrose Agar is a medium suitable to cultivate a wide variety of microorganisms, with or without blood added. The high dextrose concentration yields abundant growth in less time than other media and shortens the lag period of older cultures. Although it is a medium for general use, it is not appropriate for haemolysis studies due to the high content of dextrose.
Key features
Applications
Growth (general use).
Industry
General cultivation.
Preparation
43 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
The peptone mixture and beef extract provide nitrogen, vitamins, minerals and amino acids essential for growth. Dextrose is the fermentable carbohydrate, providing carbon and energy. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Bacteriological agar is the solidifying agent.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 7.3 ± 0.2 |
| Amount per litre | 43 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Bacteriological agar | 15 |
| Beef extract | 3 |
| Dextrose | 10 |
| Peptone mixture | 10 |
| Sodium chloride | 5 |
Preparation and use
Preparation
Suspend 43 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
- Inoculate and incubate at 35 ± 2 °C for 18-48 hours.
- The addition of 5% defibrinated blood allows the isolation of many fastidious bacteria, including Haemophilus and Neisseria.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-48 h.
| Microorganism | Specification |
|---|---|
| Neisseria meningitidisATCC 13090 | Good growth |
| Streptococcus pyogenesATCC 19615 | Good growth |
| Staphylococcus aureusATCC 25923 | Good growth |
Bibliography
- Recommended Methods for the Microbiological Examination of Foods APHA Inc., New York.
- Compendium of Methods for the Microbiological Examination of Food. 3rd edition APHA 1992.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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