Direct RT-PCR SYBR Mix SuperSens 2.0 UDG

RT-PCR and RT-qPCR mixes

Direct RT-PCR SYBR Mix SuperSens 2.0 UDG

One-step RT-qPCR SYBR Green I mix for direct RT-PCR with UDG carry-over protection

Cat. No.: DB-1324
Category: RT-PCR and RT-qPCR mixes
Formats: 2 formats
Manufacturer: DIANA Biotechnologies
Documents: Instructions for use (IFU), PDFsign in to download

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Cat. No.	Format	Price
DB-1324-100rxns	100 reactions
DB-1324-1000rxns	1,000 reactions

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Description

Ready-to-use 2X one-step RT-PCR master mix with SYBR Green I included, Direct technology for direct RT-PCR without RNA extraction and integrated uracil-DNA glycosylase and dUTP for carry-over contamination control. Contains Ultima H RNase Inhibitor for full RNA protection.

Key features

Direct technology

Direct one-step RT-PCR without RNA extraction from human saliva and serum, mammalian cell lines and viruses; single-cell gene expression analysis directly from FACS-sorted cells.

Unmatched sensitivity and robustness

Ultrasensitive detection down to ~1 copy of RNA. Resistance to PCR inhibitors enables direct RNA detection from biological matrices.

Thermostable and specific reverse transcriptase

Warm-start Script Reverse Transcriptase performs optimally at up to 60 °C, ensuring exceptional specificity.

Exceptional stability from fridge to bench

Maintains performance for at least 4 weeks at 4 °C. Probe mixes retain full sensitivity for up to 24 hours at 25 °C, even when mixed with primers and probes.

User-friendly single-component formulation

2X ready-to-use one-step RT-PCR mix: add primers, template and water.

SYBR Green I included

No separate dye needed for intercalating-dye RT-qPCR and melting curve analysis.

Effective contamination control with UDG

Contains thermolabile uracil-DNA glycosylase and an optimized ratio of dUTP and dTTP, removing carry-over contamination without compromising PCR efficiency.

Applications

Direct one-step RT-qPCR without RNA extraction
Single-cell gene expression analysis
Viral RNA detection from saliva and serum
Multiplex RT-qPCR

Technical details

Key proteins	SuperSens Taq DNA Polymerase, Script Reverse Transcriptase, Ultima H RNase Inhibitor
Hot-start	Antibody-based hot-start (Taq); warm-start reverse transcriptase
Format	2X ready-to-use one-step RT-PCR master mix, single component
Composition	Hot-start SuperSens Taq DNA polymerase, thermostable Script Reverse Transcriptase (engineered M-MLV), Ultima H RNase Inhibitor, dNTPs, MgCl2, enhancers and stabilizers, SYBR Green I dye, thermolabile uracil-DNA glycosylase and dUTP
Detection chemistry	SYBR Green I (included)
Reverse transcription temperature	Up to 60 °C
UDG / dUTP	UDG and dUTP included
Sensitivity	Down to ~1 copy of RNA
Sample input	Direct sample (saliva, serum, cell lines, viruses) or purified RNA
Kit content	100 rxns: 1,000 µL RT-PCR mix (2X) + 1,000 µL PCR grade water. 1,000 rxns: 10,000 µL RT-PCR mix (2X) + 10,000 µL PCR grade water.
Storage	Store at ≤ −70 °C. Up to four freeze-thaw cycles tested without loss in performance. Stable for 2 years at ≤ −70 °C and up to 4 weeks at 4 °C.
Bench stability	At least 4 weeks at 4 °C
QC assays	Functional test (RT-PCR), E. coli gDNA absence, RNase and DNase activity absence
Larger sizes	5x1,000 rxns, bulk and OEM sizes available on request