Extraction and purification
Empty Spin Columns 0.5 mL for Affinity Chromatography
Empty 0.5 mL spin columns for batch affinity purification with the resin of your choice
- Cat. No.
- S5355
- Category
- Extraction and purification
- Formats
- 1 format
- Manufacturer
Empty 500 µL polypropylene spin columns supplied pre-assembled in a 2.0 mL collection tube with lid, ready to be filled with your preferred affinity resin. They hold a 100-200 µL resin bed and up to 600 µL of lysate, wash or elution buffer per step, with short spins at 12,000-14,000 x g. They are suited to protein purification under native and denaturing conditions.
Key features
Resin of your choice
Compatible with Ni-NTA, Ni-IDA, Co-NTA and Co-IDA agaroses and other affinity matrices.
Ready to use
Each column comes inserted in a 2.0 mL collection tube with lid.
Fast protocol
Spins of about 20 seconds for loading, washing and elution.
Long shelf life
Stored at room temperature and stable for more than 2 years in the sealed bag.
Applications
- Batch purification of His-tagged proteins
- Small-scale expression trials
- Screening of purification conditions
- Small-volume purification
- Affinity purification under native or denaturing conditions
Technical details
| Column volume | 500 µL |
|---|---|
| Resin bed | 100-200 µL |
| Maximum load per step | 600 µL |
| Membrane | Low-binding PVDF, 0.1-0.2 µm pores |
| Centrifugal force | 12,000-14,000 x g in a fixed-angle rotor |
| Supplied with | 2.0 mL collection tube with lid for each column |
| Compatibility | Ni-NTA, Ni-IDA, Co-NTA, Co-IDA agaroses and other affinity resins |
| Storage | Room temperature (+15 °C to +30 °C) |
| Stability | More than 2 years in the sealed bag |
| Shipping | Ambient |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.