Dehydrated culture media
Eugon LT 100 Broth
For the enrichment of aerobic bacteria present in cosmetic products.
- Cat. No.
- LC-2110
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
- Also available
- Ready-to-use medium, 1 format (LC-4046)
Eugon LT 100 Broth is used as an enrichment broth to increase the initial microbial population. This medium contains ingredients that neutralize inhibitory substances present in the sample, such as lecithin and polysorbate 80, and dispersing agents, such as Ecosurf.
Key features
Applications
Enrichment of mesophilic aerobes and selective enumeration of Pseudomonas.
Industry
Cosmetics.
Preparation
37.4 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Pancreatic digest of casein and papainic digest of soy bean, obtained by the enzymatic hydrolysis of casein and soy proteins, provide nitrogen, vitamins, minerals and amino acids essential for growth of a great variety of microorganisms. Glucose is the main source of carbohydrates. Papainic digest of soy bean also contains natural sugars which promote bacterial growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Sodium sulfite neutralizes aldehydes. Lecithin and polysorbate 80 neutralize inhibiting substances present in the sample. Ecosurf acts as a dispersing agent.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber |
| Final pH (25 °C) | 7.0 ± 0.2 |
| Amount per litre | 37.4 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Glucose | 5.5 |
| L-Cystine | 0.7 |
| Lecithin | 1 |
| Pancreatic digest of casein | 15 |
| Papainic digest of soy bean | 5 |
| Polysorbate 80 | 5 |
| Sodium chloride | 4 |
| Sodium sulfite | 0.2 |
| Ecosurf-EH9 | 1 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 37.4 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Distribute into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
- Prepare the initial suspension and disperse in the broth.
- Incubate at 32.5 ± 2.5 °C for at least 20 hours, with a maximum of 72 hours.
- Subculture a defined amount of the above solution onto appropriate media depending on the microorganism to be detected: MacConkey Agar (LC-1052) for E. coli, Baird Parker Agar (LC-1100) for S. aureus, Cetrimide Agar (LC-1102) for P. aeruginosa and Sabouraud Dextrose Agar + Chloramphenicol for C. albicans.
Quality control
Microbiological test
Incubation conditions: 32.5 ± 2.5 °C / 20-72 h.
| Microorganism | Specification |
|---|---|
| Staphylococcus epidermidisATCC 12228 | Good growth |
| Salmonella typhimuriumATCC 14028 | Good growth |
| Escherichia coliATCC 25922 | Good growth |
| Staphylococcus aureusATCC 25923 | Good growth |
| Pseudomonas aeruginosaATCC 27853 | Good growth |
Bibliography
- ISO 21149 Cosmetics - Microbiology - Detection and counting of mesophilic aerobic bacteria.
- ISO 21150 Cosmetics - Microbiology - Detection of Escherichia coli.
- ISO 16212 Cosmetics - Microbiology - Counting of yeast and molds.
- ISO 18415 Cosmetics - Microbiology - Detection of specific and non-specific microorganisms.
- ISO 22718 Cosmetics - Microbiology - Detection of Staphylococcus aureus.
- ISO 22717 Cosmetics - Microbiology - Detection of Pseudomonas aeruginosa.
- ISO 18416 Cosmetics - Microbiology - Detection of Candida albicans.
- ISO 21149 Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria. Amendment 1.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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Frequently asked questions
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