Active ingredientIndigo CarmineCat. No. P-I040-001CAS 860-22-0
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Impurity or related compound · Indigo Carmine family
Pharmaceutical analytical standardIndigo Carmine Impurity 1
Synonyms: Disodium 2-(1,3-dihydro-3-oxo-7-sulphonato-2H-indol-2-ylidene)-3-oxoindoline-5-sulphonate, Disodium 3,3'-dioxo-1,1',3,3'-tetrahydro-2,2'-biindole-5,7'-disulfonate, Indigo Carmine 5,7'-Isomer, Disodium indigo-5,7'-disulfonate
- Cat. No.
- P-I040-004
- CAS
- 27414-68-2
- Alternative CAS
- 54947-75-0 (free acid)
- Formula
- C16H8N2Na2O8S2
- Molecular weight
- 466.35 g/mol
- Category
- Impurity or related compound
Family: Indigo Carmine
A blue dye obtained by sulfonating indigo: it is the food additive E132 and, injected into a vein, it helps surgeons highlight the urinary tract. In the United States it has been approved for medical use since 2022.
Did you know?It is one of the few blue food colourings and also a pH indicator: in solution it is blue at pH 11.4 and turns yellow at pH 13.
The two levels compared
| Level 1 Mass balance | Level 2 Mass balance + qNMR | |
|---|---|---|
| Identity (IR, MS, 1H and 13C-NMR; peptides: ESI-MS, MALDI-TOF and IR) | ✓ | ✓ |
| HPLC purity (min. 95%) | ✓ | ✓ |
| Content by mass balance (min. 90%; peptides: for information only)* | ✓ | ✓ |
| Content verified by qNMR (small molecules only) | · | ✓ |
| Suitable to qualify it as an in-house primary standard (ICH Q7) | · | ✓ |
* Mass balance is performed on every molecule. The only exceptions are cases where the chemistry of the molecule prevents it, for example oils and highly volatile substances, on which some of the required measurements cannot be made: in those cases the CoA states how the content was assigned.
What you receive
The analytical standard
Shipped under controlled temperature when the molecule requires it.
Certificate of analysis (CoA)
For every lot: identity, purity and assay, with the supporting spectra and chromatograms.
Safety data sheet (SDS)
Sent with every order.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
More products in the Indigo Carmine family
Active ingredientIndigo CarmineCat. No. P-I040-001CAS 860-22-0
NitrosamineN-Nitroso Indigo CarmineCat. No. P-I040-003
ImpurityIsatin-5-Sulfonic Acid Sodium Salt DihydrateCat. No. P-I040-005CAS 207399-16-4
Active ingredientPigment Blue 63Cat. No. P-I040-006CAS 16521-38-3
ImpuritySodium indigo sulfonateCat. No. P-I040-007CAS 27414-69-3Frequently asked questions
Is mass balance always performed?
Yes, on every molecule. The only exceptions are cases where the chemistry of the molecule prevents it: for example oils and highly volatile substances, on which some measurements (such as thermogravimetry) cannot be made. In those cases the CoA states which method was used to assign the content.
Can I ask for a different quantity?
Yes. The quantities are only suggestions: choose "Other" and enter the milligrams or grams you need.
Which level should I choose?
Level 1 is enough for most routine testing. Level 2 adds an independent qNMR content value and is meant for labs that need to qualify an in-house primary standard. For peptides and similar molecules, Level 2 is not available.
When will I get the quote?
Within 24 working hours, with price, availability and delivery time.
Need a molecule you cannot find?
Custom synthesis of impurities, metabolites and labelled compounds, with the same characterisation level.