Ready-to-use media
Lock&Block TSA (Trypticasein Soy Agar) TLHTh. Triple Wrapped. Irradiated EP/USP/ISO
General purpose solid medium containing animal and plant peptone, and neutralisers, according to Pharmacopoeial Harmonised Method and ISO Standards.
- Cat. No.
- LC-4575
- Category
- Ready-to-use media
- Format
- 30 Rodac contact plates, 65 mm
- ISO 17025 quality control
- Yes
- Storage
- 15 to 25 °C
- Minimum guaranteed shelf life
- 3 months
- Standards
- European PharmacopoeiaISO 11133ISO 11731ISO 18415ISO 21149ISO 21150ISO 22717ISO 22718ISO 22964ISO 9308USP
TSA is a widely used medium containing two peptones which support the growth of a wide variety of organisms, even that of very fastidious ones such as Neisseria, Listeria, Brucella, etc. It is frequently used for routine diagnostic purposes due to its reliability and its easily reproducible results.
Key features
Applications
Enumeration with neutralizers (general use).
Industry
Cosmetics, food, water.
Standards
European Pharmacopoeia, ISO 11133, ISO 11731, ISO 18415, ISO 21149, ISO 21150, ISO 22717, ISO 22718, ISO 22964, ISO 9308, USP.
Presentation
30 irradiated contact plates, triple wrapping, with 15 ± 2 mL.
Description and technique
Description
The addition of the neutralizing agents TLHTh (Tween 80 - Lecithin - Histidine - Sodium Thiosulphate) may inactivate a variety of disinfectants.
- The combination of lecithin, polysorbate 80 and histidine neutralizes aldehydes and phenolic compounds.
- The combination of lecithin and polysorbate 80 neutralizes the quaternary ammonium compounds.
- The polysorbate 80 neutralizes hexachlorophene and mercurial derivatives.
- Sodium thiosulphate neutralizes halogen compounds.
- Lecithin neutralizes chlorhexidine.
- Histidine neutralizes formaldehyde.
Technique
Collect, dilute and prepare samples and volumes as required according to specifications, directives, official standard regulations and/or expected results.
This medium is also well suited for air environmental sampling (total compatibility with most commercially available air samplers) or for other types of environmental sampling (fingers or gloves of operators, swab streaking, etc.).
Spread the plates by streaking methodology or by spiral method.
The inoculated plates are incubated at 30-35 °C for 24-72 h (bacteria) and 3-5 days for fungi (yeasts and molds). Examined daily.
(Incubation times greater than those mentioned above or different incubation temperatures may be required depending on the sample, on the specifications, etc. This medium can be inoculated directly or after enrichment broth.)
After incubation, enumerate all the colonies that have appeared onto the surface of the agar.
Each laboratory must evaluate the results according to their specifications.
Calculate total microbial count per mL of sample by multiplying the average number of colonies per plate by the inverse dilution factor if a diluted sample was streaked. Report results as colony forming units (CFU) per mL or g along with incubation time and temperature.
The lid can be used to lock the plate in two positions after taking the sample:
- AIR: lid closed, but leaving certain movement, for AEROBIC and ANAEROBIC incubations.
- CLOSE: lid completely closed. Better for transport, avoiding risk of contamination due to its possible opening during the transport.
Attention: Plates are used for monitoring the microbiological contamination of surface and air inside cleanrooms, isolators, RABS, food industries and hospitals. The double/triple irradiated wrapping ensures that the package itself doesn't contaminate the environment as the first wrapper is removed just before entering the clean area.
Wrapping resistant to hydrogen peroxide vapor penetration.
Presentation
| Presentation | 30 irradiated contact plates, triple wrapping |
|---|---|
| Filling | 15 ± 2 mL |
| Packaging | 1 box with 3 x 10 plates BOPP plastic bags (triple wrapping) with stacks of 5 plates inside. Every pack exhibits an irradiation indicator stuck on the side of the bag (8-14 kGy). Lateral labelling. Lockable Petri lid. |
Composition
| Ingredient | g/L |
|---|---|
| Peptone from casein | 15.0 |
| Soya peptone | 5.00 |
| Sodium chloride | 5.00 |
| Histidine | 1.00 |
| Lecithin | 0.70 |
| Polysorbate 80 | 5.00 |
| Sodium thiosulfate | 0.50 |
| Agar | 15.0 |
Quality control
Physical/chemical control
| Color | Straw-coloured yellow |
|---|---|
| pH | 7.3 ± 0.2 at 25 °C |
Microbiological control
Growth Promotion Test 50-100 CFU according to harmonized pharmacopoeial monographs and test methods and ISO 11133:2014/Amd 1:2018.
Analytical methodology according to ISO 11133:2014/Amd 1:2018; A2:2020.
Aerobiosis. Incubation at 30-35 °C. Read after 18-24 h to 72 h for bacteria and 3-5 days for fungi.
| Microorganism | Growth |
|---|---|
| Escherichia coliATCC 8739, WDCM 00012 | Good (≥ 70%) |
| Staphylococcus aureusATCC 6538, WDCM 00032 | Good (≥ 70%) |
| Bacillus subtilisATCC 6633, WDCM 00003 | Good (≥ 70%) |
| Candida albicansATCC 10231, WDCM 00054 | Good (≥ 70%) |
| Aspergillus brasiliensisATCC 16404 | Good (≥ 70%) |
| Ps. aeruginosaATCC 9027, WDCM 00026 | Good (≥ 70%) |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ATLAS, R.M. & L.C. PARKS (1993) Handbook of Microbiological Media. CRC Press, Inc. London.
- COLIPA (1997) Guidelines on Microbial Quality Management (MQM). Brussels.
- DOWNES, F.P. & K. ITO (2001) Compendium of Methods for the Microbiological Examination of Food, 4th ed, ASM, Washington D.C.
- EUROPEAN PHARMACOPOEIA 10.0 (2020) 10th ed. § 2.6.13. Microbiological examination of non-sterile products: Test for specified microorganisms. Harmonised Method. EDQM. Council of Europe. Strasbourg.
- FDA (Food and Drug Administration) (1998) Bacteriological Analytical Manual. 8th ed. Rev. A. AOAC International. Gaithersburg. MD.
- HORWITZ, W. (2000) Official Methods of Analysis of AOAC INTERNATIONAL, 17th ed. Gaithersburg, MD. USA.
- ISO 9308-1 Standard (2000) Water Quality. Detection and enumeration of E. coli and coliform bacteria. Membrane filtration method.
- ISO 11731 Standard (2017) Water Quality - Enumeration of Legionella.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- ISO 18415 Standard (2017) Cosmetics - Microbiology - Detection of specified and non-specified microorganisms.
- ISO 21149 Standard (2017) Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria.
- ISO 21150 Standard (2015) Cosmetics - Microbiology - Detection of Escherichia coli.
- ISO 22717 Standard (2015) Cosmetics - Microbiology - Detection of Pseudomonas aeruginosa.
- ISO 22718 Standard (2015) Cosmetics - Microbiology - Detection of Staphylococcus aureus.
- ISO 22964 (2017) Microbiology of the food chain - Horizontal method for the detection of Cronobacter spp.
- PASCUAL ANDERSON, Mª R. (1992) Microbiología Alimentaria. Díaz de Santos S.A., Madrid.
- USP 33 - NF 28 (2011) <62> Microbiological examination of non-sterile products: Test for specified microorganisms. Harmonised Method. USP Corp. Inc. Rockville. MD. USA.
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The product
Shipped under controlled temperature when the product requires it.
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