Ready-to-use media
Luria Agar (Miller’s LB Agar)
Solid medium of general use, recommended for studies of molecular genetics with Escherichia coli.
- Cat. No.
- LC-996
- Category
- Ready-to-use media
- Format
- 20 plates, 90 mm
- Storage
- 2 to 14 °C
- Minimum guaranteed shelf life
- 1 month
This solid medium corresponds to the basic Lennox agar formulation, modified by Miller, which increased the concentration of sodium chloride and added agar.
Key features
Applications
Preparation and recovery of Escherichia coli competent cells.
Industry
Molecular biology.
Presentation
20 prepared plates, 90 mm, with 21 ± 2 mL.
Packaging
1 box with 2 packs of 10 plates/pack. Single cellophane.
Description and technique
Technique
Dilute and prepare samples and volumes as necessary according to specific protocols, established regulations, official directives and/or expected results. Each technician must evaluate the results according to the specifications established in his laboratory.
Presentation
| Presentation | 20 prepared plates, 90 mm |
|---|---|
| Filling | 21 ± 2 mL |
| Packaging | 1 box with 2 packs of 10 plates/pack. Single cellophane. |
Composition
| Ingredient | g/L |
|---|---|
| Casein peptone | 10.0 |
| Yeast extract | 5.0 |
| Sodium chloride | 10.0 |
| Agar | 15.0 |
Quality control
Physical/chemical control
| Color | Straw-coloured yellow |
|---|---|
| pH | 7.2 ± 0.2 at 25 °C |
Microbiological control
Inoculate: practical range 100 ± 20 CFU; min. 50 CFU (productivity).
Aerobiosis. Incubation at 35 ± 2 °C. Reading at 24 hours.
| Microorganism | Growth |
|---|---|
| Escherichia coliATCC 11775, WDCM 00090 | Good (≥ 70%) |
| Escherichia coliATCC 8739, WDCM 00012 | Good (≥ 70%) |
| Escherichia coliATCC 25922, WDCM 00013 | Good (≥ 70%) |
| Escherichia coliATCC 35218 | Good (≥ 70%) |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ATLAS, R.M., L.C. PARKS (1993) Handbook of Microbiological Media. CRC Press, Inc. London.
- AUSUBEL, F.M., R. BRENT, R.E. KINGSTON, D.D. MOORE, J.G. SEIDMAN, J.A. SMITH & K. STRUHL (1994) Current protocols in molecular biology. Greene Pub. Assoc. Inc. Brooklyn, N.Y.
- GHERNA, R., P. PIENTA, R. COTE (Eds.) 1992. ATCC Catalogue of Bacteria and Bacteriophages. Media #1065, #1226, #1226, #1235, #1236, #1315, #1364. American Type Culture Collection. Rockville MD. USA.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- LENNOX, E.S. (1955) Transduction of linked genetic character of the host bacteriophage P1. Virology 1:190-206.
- LURIA, S.E. & J.W. BURROUS (1955) Hybridization between Escherichia coli and Shigella. J. Bacteriol. 74:461-476.
- MILLER, J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor Laboratory. Cold Spring Harbor, N.Y.
- SAMBROOK, J., E.F. FITSCH & T. MANIATIS (1989) Molecular cloning: A laboratory manual. 2nd ed. Cold Spring Harbor Laboratory. Cold Spring Harbor, N.Y.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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