Dehydrated culture media
Marine Broth
For the isolation and enumeration of heterotrophic marine bacteria.
- Cat. No.
- LC-1217
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Marine Broth is similar to Marine Agar (LC-1059), lacking the agar, but containing all the nutrients necessary to cultivate the majority of marine bacteria.
Key features
Applications
Selective enumeration of heterotrophic marine bacteria.
Preparation
40.20 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Since the marine environment has environmental conditions completely different to those of other environments, its microflora is also very different. Marine microorganisms are capable of surviving at very low temperatures and in high salinity levels.
Both Marine Agar and Marine Broth are prepared according to ZoBell, containing almost double the mineral content of sea water. The high salt content helps to simulate sea water. Bacteriological peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group. Bacteriological agar is the solidifying agent.
Technical specifications
| Solubility | May present a light precipitate |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 7.6 ± 0.2 |
| Amount per litre | 40.20 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Ammonium nitrate | 0.0016 |
| Bacteriological peptone | 5 |
| Boric acid | 0.022 |
| Calcium chloride | 1.8 |
| Disodium phosphate | 0.008 |
| Magnesium chloride anhydrous | 8.8 |
| Potassium bromide | 0.08 |
| Potassium chloride | 0.55 |
| Sodium bicarbonate | 0.16 |
| Sodium chloride | 19.4 |
| Sodium fluoride | 0.0024 |
| Sodium silicate | 0.004 |
| Sodium sulfate | 3.24 |
| Strontium chloride | 0.034 |
| Yeast extract | 1 |
| Ferric citrate | 0.1 |
Preparation and use
Preparation
Suspend 40.20 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes. The prepared medium should be stored at 2-8 °C.
Instructions for use
Dispense 50 mL of the broth in 250 mL Erlenmeyer flasks. Inoculate and incubate at 20-25 °C for 24-72 hours.
Quality control
Microbiological test
Incubation conditions: 20-25 °C / 24-72 h.
| Microorganism | Specification |
|---|---|
| Vibrio harveyiATCC 14126 | Good growth |
| Vibrio alginolyticusATCC 17749 | Good growth |
| Vibrio parahaemolyticusATCC 17802 | Good growth |
Bibliography
- J. Marine Research N:42. 1941.
- Limnology and Oceanography 5:78, 1960.
- ZoBell, C.E. 1941. Studies on Marine Bacteria. I. The cultural requirements of heterotrophic aerobes. J. Mar. Res. 4:42-75.
- Buck, J.D., and R.C. Cleverdon. 1960. The spread plate as a method for the enumeration of marine bacteria. Limnol. Oceanogr.
- Weiner, R.M., A.M. Segall, and R.R. Colwell. 1985.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
Other products in the same category
Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.