Ready-to-use media
Modified Scholten Agar ISO
Solid culture medium used as a basal layer in the enumeration of somatic coliphages in samples of any type of water, sediment and sludge, according to ISO 10705-2 Standard.
- Cat. No.
- LC-5247
- Category
- Ready-to-use media
- Format
- 10 x 200 mL flasks
- Storage
- 8 to 25 °C
- Minimum guaranteed shelf life
- 4 months
- Standards
- ISO 10705
Scholten's Modified Agar is described in ISO 10705-2:2000 Water Quality. Detection and enumeration of bacteriophages. Part 2: Count of somatic coliphages.
Key features
Applications
Non-selective enumeration of somatic coliphages.
Industry
Water.
Standards
ISO 10705.
Presentation
10 prepared bottles, 250 mL, with 200 ± 5 mL.
Description and technique
It is used as a culture medium for the basal layer of the plates for the counting of bacteriophages. For details about the technique and the procedure, the technician is referred to the aforementioned regulations.
The base medium is melted and cooled to a temperature between 45-50 °C. Add the calcium chloride solution under aseptic conditions. Mix carefully and distribute into plates.
Note: the solid media can be melted in different ways: autoclave, bath and, if the customer considers it appropriate, also the microwave. Whenever the microwave option is chosen, it is necessary to take certain safety measures to avoid breaking of the containers, such as loosening the screw cap and putting the bottle or tube in a water bath in the microwave. The fusion temperature and time will depend on the shape of the container, the volume of medium and the heat source. Avoid overheating as well as prolonged heating periods.
Presentation
| Presentation | 10 prepared bottles, 250 mL |
|---|---|
| Filling | 200 ± 5 mL |
| Packaging | 1 box with 10 bottles 250 mL. Injectable cap: plastic screw inner cap. The use of syringe needles with a diameter greater than 0.8 mm is not recommended. |
Composition
| Ingredient | g/L |
|---|---|
| Tryptone | 10.0 |
| Yeast extract | 3.00 |
| Meat extract | 12.0 |
| Sodium chloride | 3.00 |
| Magnesium chloride (anhydrous) | 0.60 |
| Sodium carbonate | 0.75 |
| Agar | 15.00 |
Quality control
Physical/chemical control
| Color | Yellowish |
|---|---|
| pH | 7.2 ± 0.5 at 25 °C |
Microbiological control
Inoculate: practical range 100 ± 20 CFU; min. 50 CFU (productivity).
Analytical methodology according to ISO 11133:2014/Amd 1:2018; A2:2020.
Aerobiosis. Incubation at 35 ± 2 °C. Reading at 24 hours.
| Microorganism | Growth |
|---|---|
| Escherichia coliATCC 43888 | Good (≥ 70%) |
| Escherichia coliATCC 700078 (WG5) | Good (≥ 70%) |
| Escherichia coliATCC 13706 | Good (≥ 70%) |
Sterility control
Incubation 48 h at 30-35 °C and 48 h at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- GRABOW W.O.K. & P. COUBOROUGH (1986) Practical direct plaque assay for coliphages in 100 mL samples of drinking water. Appl. Environ. Microbiol. 52:430-433.
- HAVELAAR, A.H. & W.M. HOGEBOOM (1983) Factors affecting the enumeration of coliphages in sewage and in sewage-polluted waters. Antonie van Leeuwenhoek 49:387-397.
- KENNEDY, J.E., G. BITTON & J.L. OBLINGER (1985) Comparison of Selective Media for Assay of Coliphages in Sewage Effluent and Lake Water. Appl. Environ. Microbiol. 49:1:33-36.
- MUNIESA, M. & J. JOFRE (1998) Abundance in Sewage of Bacteriophages That Infect Escherichia coli O157:H7 and That Carry the Shiga Toxin 2 Gene. Appl. Environ. Microbiol. 64:7:2443-24.
- UNE-EN ISO Standard 10705-2:2001 Calidad del agua. Detección y recuento de bacteriófagos. Parte 2: Recuento de colifagos somáticos. International Standardization Organization. Geneva. Switzerland.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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