Ready-to-use media
Potato Dextrose Agar EP/USP
Culture medium used for the detection and enumeration of fungi in food, dairy products and other samples according to the Pharmacopeial Harmonised Method.
- Cat. No.
- LC-849
- Category
- Ready-to-use media
- Format
- 20 plates, 90 mm
- ISO 17025 quality control
- Yes
- Storage
- 2 to 14 °C
- Minimum guaranteed shelf life
- 1 month
- Standards
- European PharmacopoeiaUSP
- Also available
- Dehydrated medium, 1 format (LC-1022)
Potato Dextrose Agar is a weakly selective medium for fungi due to its high sugar content and acidic pH. Pigment production and aerial mycelium development are enhanced by the potato peptone, especially in Fusarium, Aspergillus and Penicillium species.
Key features
Applications
Selective enumeration of yeasts and molds.
Industry
Clinical, final product quality control, food, pharmaceutical/veterinary.
Standards
European Pharmacopoeia, USP.
Presentation
20 prepared plates, 90 mm, with 21 ± 2 mL.
Description and technique
Technique
For plate inoculation, follow the laboratory's standard methods or the applicable norms (spiral plating method, ecometric methods, streak plating, dilution banks, spread plating with Drigalski rod, etc.).
Incubated for 48 h to 5-7 days at 20-25 °C to permit the complete development of the fungal (mold and yeast) colonies.
Presentation
| Presentation | 20 prepared plates, 90 mm |
|---|---|
| Filling | 21 ± 2 mL |
| Packaging | 1 box with 2 packs of 10 plates/pack. Single cellophane. |
Composition
| Ingredient | g/L |
|---|---|
| Potato peptone (1) | 4.00 |
| Glucose | 20.00 |
| Agar | 15.00 |
(1) Equivalent to 200 g infusion from potatoes.
Quality control
Physical/chemical control
| Color | Yellowish |
|---|---|
| pH | 5.6 ± 0.2 at 25 °C |
Microbiological control
Inoculate: 50-100 CFU (productivity) according to harmonized Eur. Pharmacopoeia.
Aerobic. Incubation at 22.5 ± 2 °C up to 5 days (moulds and yeast).
| Microorganism | Growth |
|---|---|
| Aspergillus brasiliensisATCC 16404, WDCM 00053 | Good (≥ 70%) |
| Candida albicansATCC 10231, WDCM 00054 | Good (≥ 70%) |
| S. cerevisiaeATCC 9763, WDCM 00058 | Good (≥ 70%) |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ATLAS R.M. (1995) Handbook of Microbiological Media for the Examination of Food. CRC Press. Boca Raton. Florida. USA.
- EUROPEAN PHARMACOPOEIA 8.0 (2014) 8th ed. § 2.6.13. Microbiological examination of non-sterile products: Test for specified microorganisms. Harmonised Method. EDQM. Council of Europe. Strasbourg.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- RICHARDSON, G.H. (1985) Standard Methods for the examination of dairy products 15th ed. APHA. Washington.
- USP 33 - NF 28 (2011) <62> Microbiological examination of non-sterile products: Test for specified microorganisms. Harmonised Method. USP Corp. Inc. Rockville. MD. USA.
- VANDERZANT, C. & D.F. SPLITTSTOESSER (1992) Compendium of methods for the microbiological examination of foods. 3rd ed. APHA. Washington.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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How long does delivery take?
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