Fluorescent microspheres10 µmBlue Fluorescent Polymer Microspheres 10 µm 1% suspensionCat. No. 51100B-50Pack size 50 mLPrice 
Fluorescent microspheres
Red Fluorescent Polymer Microspheres 10 µm 1% suspension
- Cat. No.
- 51100R-01
- Diameter
- 10 µm
- Pack size
- 1 g
- Type
- Certified reference material
- Manufacturer
Technical data
| Nominal diameter | 10 µm |
|---|---|
| Colour | red |
| Excitation | 450-570 nm |
| Emission | 550-660 nm |
| Form | powder |
| Material | Polystyrene with fluorescent dye incorporated in the matrix during polymerisation |
| Colours | Blue, green, red |
| Blue: excitation / emission | 380-450 nm / 440-520 nm |
| Green: excitation / emission | 400-490 nm / 470-560 nm |
| Red: excitation / emission | 450-570 nm / 550-660 nm |
| Diameters | 0.1; 0.5; 1; 5; 10; 15; 20 µm |
| Packs | Suspension 3 mL or 50 mL; powder 1 g (from 5 to 20 µm) |
| Fluorescence | High intensity, low particle-to-particle variation, no dye leakage |
| Storage | Room temperature |
Description
Polystyrene microspheres with the fluorescent dye incorporated during polymerisation, so no dye leakage, in three colours: blue, green and red. Fluorescence is bright and very uniform from particle to particle, and it does not degrade in water or when the microspheres are dispersed in air. They are detected with flow cytometers, epifluorescence and confocal microscopes, fluorescence spectrophotometers and even with a black light, directly in the sample or collected on a membrane filter. 1% aqueous suspension in 3 or 50 mL bottles, or 1 g of dry powder for diameters from 5 to 20 µm.
Applications
- Flow cytometer calibration and set-up
- Fluorescence microscopy and reference particles for confocal microscopy
- Fluorescence instrument monitoring
- Filter media and filtration system testing
- Vial and container cleaning studies
- Flow tracing and fluid mechanics
- Centrifugation and sedimentation studies
What you receive
Certificate
Lot certificate with certified diameter or concentration, traceable to NIST and SI.
Import and shipping
Import, customs and Italian invoice handled by VitaSan Labs, tracked shipping.
Technical support
We help you choose diameter, concentration and format for your instrument and method.
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Are the standards traceable?
Yes: mean diameter, standard deviation and uncertainty are traceable to NIST and the International System of Units (SI) and stated on the certificate of every lot. The ISO/IEC 17025 series is certified by an accredited metrology laboratory.
Which control do I need for USP <788>?
For subvisible particle counting in injectables, use the PharmaCount controls: the USP <788> set for routine verification, the 3M and PC sets for single diameters. For USP <729> there is PharmaCount BiCal.
Can I order a diameter I do not see?
Yes: other diameters, concentrations and dyes are available on request. Tell us the value you need and we will send a quote.
How do I see the prices?
List prices per pack, excluding VAT, are shown after signing in to the customer area. With the quote request we confirm price, availability and delivery time.