RT-PCR and RT-qPCR mixesRT-PCR Probe Mix SuperSens 2.0Cat. No. DB-1312Formats 2 formats
RT-PCR and RT-qPCR mixes
RT-PCR Probe Mix SuperSens 2.0 dUTP
One-step RT-qPCR probe mix with dUTP, UDG-free
- Cat. No.
- DB-1314
- Category
- RT-PCR and RT-qPCR mixes
- Formats
- 2 formats
- Manufacturer
- DIANA Biotechnologies
- Documents
- Instructions for use (IFU), PDFsign in to downloadInstructions for use (IFU), PDF233 KB
Description
Ready-to-use 2X one-step RT-PCR master mix for hydrolysis (TaqMan) probes containing dUTP but no uracil-DNA glycosylase: amplicons carry uracil so they can be degraded by UDG in your own decontamination workflow. SuperSens technology with hot-start SuperSens Taq and thermostable Script Reverse Transcriptase.
Key features
Unmatched sensitivity and robustness
Ultrasensitive detection down to ~1 copy of RNA. Resistance to PCR inhibitors enables direct RNA detection from biological matrices.
Thermostable and specific reverse transcriptase
Warm-start Script Reverse Transcriptase performs optimally at up to 60 °C, ensuring exceptional specificity.
Exceptional stability from fridge to bench
Maintains performance for at least 4 weeks at 4 °C. Probe mixes retain full sensitivity for up to 24 hours at 25 °C, even when mixed with primers and probes.
User-friendly single-component formulation
2X ready-to-use one-step RT-PCR mix: add primers, template and water.
dUTP-containing, UDG-free
dUTP incorporated in amplicons for downstream UDG-based decontamination in your own workflow; no UDG in the mix.
Applications
- One-step RT-qPCR
- Gene expression analysis
- Viral RNA detection
- Multiplex RT-qPCR
Technical details
| Key proteins | SuperSens Taq DNA Polymerase, Script Reverse Transcriptase, RNase Inhibitor Bovine |
|---|---|
| Hot-start | Antibody-based hot-start (Taq); warm-start reverse transcriptase |
| Format | 2X ready-to-use one-step RT-PCR master mix, single component |
| Composition | Hot-start SuperSens Taq DNA polymerase, thermostable Script Reverse Transcriptase (engineered M-MLV), RNase Inhibitor Bovine, dNTPs, MgCl2, enhancers and stabilizers, dUTP |
| Detection chemistry | Hydrolysis (TaqMan) probes; suitable for multiplex RT-qPCR |
| Reverse transcription temperature | Up to 60 °C |
| UDG / dUTP | dUTP included, UDG-free |
| Sensitivity | Down to ~1 copy of RNA |
| Sample input | Purified RNA; selected biological samples directly, optionally after Lysis Buffer B |
| Kit content | 100 rxns: 1,000 µL RT-PCR mix (2X) + 1,000 µL PCR grade water. 1,000 rxns: 10,000 µL RT-PCR mix (2X) + 10,000 µL PCR grade water. |
| Storage | Store at −18 °C to −25 °C. Up to four freeze-thaw cycles tested without loss in performance. Stable for 2 years at ≤ −18 °C and up to 4 weeks at 4 °C. |
| Bench stability | At least 4 weeks at 4 °C; up to 24 hours at 25 °C with primers and probes |
| QC assays | Functional test (RT-PCR), E. coli gDNA absence, RNase and DNase activity absence |
| Larger sizes | 5x1,000 rxns, bulk and OEM sizes available on request |
Performance data
Manufacturer information
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.