Dehydrated culture media
Sabouraud Dextrose Agar 2%
For the cultivation of dermatophytes.
- Cat. No.
- LC-1166
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Sabouraud Dextrose Agar 2% is recommended for the cultivation of dermatophytes. The fungi colonies can be observed macro- and microscopically.
Key features
Applications
Selective isolation of dermatophytes.
Industry
Cosmetics, food.
Preparation
47 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
To prepare a selective culture medium, aseptically add cycloheximide, penicillin and streptomycin, since it has been demonstrated that the basic agar, fortified by three antibiotics, considerably improves the isolation of pathogenic fungi from heavily contaminated sources.
Dextrose is the fermentable carbohydrate providing carbon and energy. Peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Bacteriological agar is the solidifying agent.
The high dextrose concentration makes this medium selective for fungi. The low pH of approximately 5.6 is appropriate for the growth of dermatophytes, as well as being slightly inhibitory to contaminating bacteria in specimens.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 5.6 ± 0.2 |
| Amount per litre | 47 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Bacteriological agar | 17 |
| Dextrose | 20 |
| Peptone | 10 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 47 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Distribute and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
- Use standard procedures to inoculate the sample material.
- Incubate the Sabouraud Dextrose Agar 2% plates for up to 7 days at 28 °C in aerobic conditions.
Quality control
Microbiological test
Incubation conditions: 28 ± 2 °C / 7 days.
| Microorganism | Specification |
|---|---|
| Candida albicansATCC 10231 | Good growth |
| Aspergillus brasiliensisATCC 16404 | Good growth |
| Trichophyton mentagrophytesATCC 24957 | Good growth |
| Trichophyton rubrumCECT 2794 | Moderate growth |
Bibliography
- Georg, L.K., Ajello, L. and Papageorge, C.: Use of cycloheximide in the selective isolation of fungi pathogenic to man.
What you receive
The product
Shipped under controlled temperature when the product requires it.
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