Ready-to-use media
Sabouraud Dextrose Agar. Triple Wrapped. Irradiated EP/USP
Solid medium for the enumeration and cultivation of fungi according to the Pharmacopeial Harmonised Method and ISO standard.
- Cat. No.
- LC-807
- Category
- Ready-to-use media
- Format
- 20 plates, 90 mm
- ISO 17025 quality control
- Yes
- Storage
- 15 to 25 °C
- Minimum guaranteed shelf life
- 1 month
- Standards
- European PharmacopoeiaISO 11133ISO 16212USP
- Also available
- Ready-to-use medium, 1 format (LC-4542)
Sabouraud Dextrose Agar is a modification of the classical Sabouraud medium for the cultivation of fungi. This new formula helps to maintain the morphology of fungi, providing a reliable medium for both cultivation and differentiation.
Key features
Applications
Cultivation and selective enumeration of yeasts and molds.
Industry
Cosmetics, food, water.
Standards
European Pharmacopoeia, ISO 11133, ISO 16212, USP.
Presentation
20 irradiated plates, 90 mm, triple wrapping, with 21 ± 1 mL.
Description and technique
Its selectivity is due to a low pH and a high glucose concentration, which together with incubation at a relatively lower temperature (20-25 °C) favours the growth of fungi while discouraging that of bacteria. The mixture of peptones employed has been selected to provide the fungi with all their nitrogen requirements.
Spread the plate by streaking methodology or by spiral method.
Each laboratory must evaluate the results according to their specifications.
Attention: Petri plates are used for monitoring the microbiological contamination of surface and air inside cleanrooms, isolators, RABS, food industries and hospitals. The double/triple irradiated wrapping ensures that the package itself doesn't contaminate the environment as the first wrapper is removed just before entering the clean area.
Wrapping resistant to hydrogen peroxide vapor penetration.
Presentation
| Presentation | 20 irradiated plates, 90 mm, triple wrapping |
|---|---|
| Filling | 21 ± 1 mL |
| Packaging | 1 box with 2 BOPP bags (triple wrapping) with 10 plates/bag. Every pack exhibits an irradiation indicator stuck on the side of the bag (8-14 kGy), with desiccant. Lateral labelling. |
Composition
| Ingredient | g/L |
|---|---|
| D(+)-Glucose | 40.0 |
| Peptone from casein | 5.0 |
| Meat peptone | 5.0 |
| Agar | 15.0 |
Quality control
Physical/chemical control
| Color | Straw-coloured yellow |
|---|---|
| pH | 5.6 ± 0.2 at 25 °C |
Microbiological control
Growth Promotion Test 50-100 CFU according to harmonized pharmacopoeial monographs and test methods and ISO 11133:2014/Amd 1:2018.
Spiral spreading: practical range 50-100 CFU (productivity).
Analytical methodology according to ISO 11133:2014/Amd 1:2018; A2:2020.
Aerobiosis. Incubation at 20-25 °C. Reading ≤ 5 days.
| Microorganism | Growth |
|---|---|
| Candida albicansATCC 10231, WDCM 00054 | Good (≥ 70%) |
| Aspergillus brasiliensisATCC 16404 | Good (≥ 70%) |
| Saccharomyces cerevisiaeATCC 9763 | Good (≥ 70%) |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- AJELLO, L. (1957) Cultural Methods for Human Pathogenic Fungi. J. Chron. Dis. 5:545-551.
- COLIPA (1997) Guidelines on Microbial Quality Management (MQM). Brussels.
- EUROPEAN PHARMACOPOEIA 10.0 (2020) 10th ed. § 2.6.13. Microbiological examination of non-sterile products: Test for specified microorganisms. Harmonised Method. EDQM. Council of Europe. Strasbourg.
- GEORGE, L.K., AJELLO, L. & PAPAGEORGE, C. (1954) Use of Cycloheximide in the Selective Isolation of Fungi Pathogenic to Man. J. Lab. Clin. Med, 44 (422-428).
- HANTSCHKE, D. (1968) Mykosen, 11, (769-778).
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- ISO 16212 Standard (2017) Cosmetics - Microbiology - Enumeration of yeast and mould.
- PAGANO, J. LEVIN, J.D. and TREJO, W. (1957-58) Diagnostic Medium for Differentiation of Species of Candida. Antibiotics Annual, 137-143.
- SABOURAUD, R. (1910) Les Teignes. Masson, Paris.
- USP 33 - NF 28 (2011) <62> Microbiological examination of non-sterile products: Test for specified microorganisms. Harmonised Method. USP Corp. Inc. Rockville. MD. USA.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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How long does delivery take?
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