Dehydrated culture media
Salmonella Shigella Broth
For the cultivation of Salmonella and Shigella.
- Cat. No.
- LC-1463
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Salmonella Shigella Broth is a selective and differential medium used to enrich Salmonella and Shigella from feces, urine, and fresh and canned foods.
Key features
Applications
Selective enrichment of Salmonella and Shigella.
Industry
Food, water.
Preparation
46.5 g in one liter of distilled water; boil for one minute, do not autoclave.
Principles and uses
Beef extract and peptone mixture provide nitrogen, vitamins, minerals and amino acids essential for growth. Lactose is the fermentable carbohydrate providing carbon and energy. Bile salts mixture, sodium citrate and brilliant green inhibit Gram-positive bacteria, most coliform bacteria and swarming Proteus spp., while allowing Salmonella spp. to grow. Neutral red is the pH indicator. Sodium thiosulfate and ferric citrate allow the detection of the H₂S-producing bacteria.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Red orange |
| Final pH (25 °C) | 7.0 ± 0.2 |
| Amount per litre | 46.5 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Beef extract | 5 |
| Bile salts | 8.5 |
| Brilliant green | 0.0003 |
| Lactose | 10 |
| Neutral red | 0.025 |
| Peptone mixture | 5 |
| Sodium citrate | 8.5 |
| Sodium thiosulfate | 8.5 |
| Ferric citrate | 1 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 46.5 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. DO NOT AUTOCLAVE. Cool to 45-50 °C and distribute into tubes.
Instructions for use
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-24 h.
| Microorganism | Specification |
|---|---|
| Shigella flexneriATCC 12022 | Good growth |
| Klebsiella aerogenesATCC 13048 | Partial inhibition |
| Salmonella enteritidisATCC 13076 | Good growth |
| Salmonella typhimuriumATCC 14028 | Good growth |
| Enterococcus faecalisATCC 19433 | Total inhibition |
| Escherichia coliATCC 25922 | Total inhibition |
| Proteus mirabilisATCC 25933 | Partial inhibition |
Bibliography
- Pub. Health Reports. 65:1075. 1950. Paper Read at Microbiological Congress, 1950. Proc. 22nd Ann. Meet. Northeastern Conf. Lab. Workers in Pullorum Disease Control Burlington, Vermont, June 20-21. 1950.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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