DNA polymerasesAptaTaq DNA PolymeraseCat. No. DB-1326Formats 3 formats
DNA polymerases
SuperSens Taq DNA Polymerase Green
Highly sensitive and specific hot-start Taq DNA polymerase, green ready-to-load formulation
- Cat. No.
- DB-1329
- Category
- DNA polymerases
- Formats
- 3 formats
- Manufacturer
- DIANA Biotechnologies
- Documents
- Instructions for use (IFU), PDFsign in to downloadInstructions for use (IFU), PDF232 KB
Description
The proprietary SuperSens Taq DNA Polymerase is the enzyme at the heart of all SuperSens 2.0 mixes. Ultra-efficient antibody-mediated hot-start ensures superior PCR specificity and sensitivity even in complex DNA mixtures and multiplexed detection, for both routine and high-demanding applications. The green formulation is supplied with PCR Buffer B Green (5X) for direct loading of PCR products on agarose gel.
Key features
Antibody hot-start
Ultra-efficient proprietary antibody-mediated hot-start ensures superior PCR specificity and sensitivity even in complex DNA mixtures and multiplexed detection.
High thermostability
Recombinant enzyme with a half-life of over 40 min at 95 °C, suitable for amplification of DNA up to 5 kb.
Three enzymatic activities
Intrinsic 5'-3' polymerase, 5'-3' exonuclease and 5' flap activities for various molecular biology applications.
All-inclusive formulation
Supplied with PCR Buffer B (5X) containing enhancers, stabilizers and optimal concentration of KCl, MgCl2 and dNTPs: no other components needed for a standard PCR.
Ready-to-load green formulation
PCR Buffer B Green (5X) also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel.
Applications
- Standard PCR
- High-yield PCR
- Colony PCR
- T/A cloning
- Genotyping
- GC-rich amplification
Technical details
| Enzyme | Recombinant Taq DNA polymerase, antibody hot-start (SuperSens Taq) |
|---|---|
| Hot-start | Antibody-based |
| Enzymatic activities | 5'-3' polymerase, 5'-3' exonuclease, 5' flap endonuclease |
| Thermostability | Half-life > 40 min at 95 °C |
| Amplicon length | Up to 5 kb |
| Supplied with | PCR Buffer B Green (5X) with KCl, MgCl2, dNTPs, enhancers and stabilizers, density reagent and two tracking dyes |
| Formulation | Green, ready-to-load |
| Storage | Store at −18 °C to −25 °C. Avoid more than 5 freeze-thaw cycles; aliquot after the first thawing if used repeatedly. |
| Applications | Standard PCR, high-yield PCR, colony PCR, T/A cloning, genotyping, GC-rich amplification, real-time PCR from cDNA, genomic or plasmid DNA |
Performance data
Manufacturer information
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
Related products
DNA polymerasesAptaTaq DNA PolymeraseCat. No. DB-1326Formats 3 formats
DNA polymerasesAptaTaq DNA Polymerase GreenCat. No. DB-1327Formats 3 formats
DNA polymerasesSuperSens Taq DNA PolymeraseCat. No. DB-1328Formats 3 formatsFrequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.