Dehydrated culture media
Trypticasein Soy Broth w/o Dextrose
For general laboratory use and to cultivate fastidious microorganisms.
- Cat. No.
- LC-1225
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Trypticasein Soy Broth w/o Dextrose is a medium very rich in nutrients for general use in microbiological laboratories.
Key features
Applications
Enrichment (general use).
Industry
Food, water.
Preparation
27.5 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
The medium is used frequently in many procedures of microbiology. For example, it is used for the isolation and sensitivity testing of all types of pathogens, and for the production of antigens for agglutination and serological tests.
Containing two peptones as rich nitrogen sources, obtained by the enzymatic hydrolysis of casein and soy proteins, this medium supports the growth of a great variety of microorganisms, including fastidious aerobes and anaerobes. Soy peptone also contains natural sugars which promote bacterial growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance, and dipotassium phosphate is a buffering agent.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Light beige |
| Color of the prepared medium | Clear amber |
| Final pH (25 °C) | 7.3 ± 0.2 |
| Amount per litre | 27.5 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Dipotassium phosphate | 2.5 |
| Pancreatic digest of casein | 17 |
| Sodium chloride | 5 |
| Papaic digest of soyabean meal | 3 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 27.5 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes. Larger quantities may require a longer sterilization time, but the temperature should not be increased.
Instructions for use
Inoculate and incubate at 35 ± 2 °C for 18-24 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-24 h.
| Microorganism | Specification |
|---|---|
| Candida albicansATCC 10231 | Good growth |
| Klebsiella aerogenesATCC 13048 | Good growth |
| Streptococcus pyogenesATCC 19615 | Good growth |
| Escherichia coliATCC 25922 | Good growth |
| Staphylococcus aureusATCC 25923 | Good growth |
| Streptococcus pneumoniaeATCC 6305 | Good growth |
Bibliography
- Gibbons and McDonald. J. Bacteriol., 80:164. 1960.
- Havens and Benham. A. Med. Tech., 23:305. 1957.
- Muey and Edward. Proc. Soc. Exper. Biol. and Med., 97:550. 1958.
- Steward and Kelly. J. Bacteriol., 77:101. 1959.
- MacFaddin, J.D. 1985. Media for isolation-cultivation-identification-maintenance of medical bacteria, p. 797. vol. 1. Williams & Wilkins, Baltimore, MD.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
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