Agaroses
Agarose D2
Used in nucleic acid and protein electrophoresis (immunoelectrophoresis and counter electrophoresis) and for the preparation of agarose beads.
- Cat. No.
- LC-803200
- Category
- Agaroses
- Formats
- 5 formats
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Agarose D2 is used in nucleic acid and protein electrophoresis (immunoelectrophoresis and counter electrophoresis) and for the preparation of agarose beads. Agarose D2 has a higher gelling temperature than Agarose D1. This characteristic provides a greater thermal stability to the gels.
Key features
Industry
Cloning, culture media for molecular biology, PCR and electrophoresis, proteomics.
Principles and uses
Some important features are:
- Extraordinary mechanical resistance for more reliable and easier handling.
- Possibility of varying pore size in accordance with particle size by modifying the gel concentration.
- Easy preparation of the gel by simple dissolution in aqueous buffers either by standard boiling or microwaving.
- Greater thermal stability due to high hysteresis (difference between gelling and melting temperatures).
- Excellent transparency of the gels.
- Excellent elasticity and flexibility of the gels.
- Great capacity for derivatization and cross-linking, which allows coupling of enzymes, antigens and other substances to the gel structure.
- Exceptionally low absorption of staining agents.
- Absence of toxicity.
Agarose D2 is used in nucleic acid electrophoresis, protein electrophoresis (immunoelectrophoresis and counterelectrophoresis) and preparation of agarose beads.
Technical specifications
| Ash | ≤ 0.4% |
|---|---|
| Clarity 1.5% (NTU) | ≤ 4 |
| Gel strength 1% (g/cm²) | ≥ 900 |
| Gel strength 1.5% (g/cm²) | ≥ 1200 |
| Gelling temperature 1.5% (°C) | 42 ± 1.5 °C |
| Melting temperature 1.5% (°C) | 87 ± 1.5 °C |
| DNase/RNase activity | None detected |
| EEO | ≤ 0.14 |
| Moisture | ≤ 10% |
| Color | White |
| Appearance | Fine, homogeneous powder |
| DNA resolution ≥ 1000 bp | Finely resolved |
| Comparative assay of different size DNA fragments | Clear and sharp bands |
| Background fluorescence assay in ethidium bromide | Very low |
| Sulphate | ≤ 0.2% |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
Other products in the same category
Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.