Agaroses
Agarose LM
Low melting / gelling temperature agarose for DNA electrophoresis (1000 bp), preparative electrophoresis and recovery of DNA fragments.
- Cat. No.
- LC-805000
- Category
- Agaroses
- Formats
- 5 formats
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Agarose Low Melting (LM) is derivatized by organic synthesis which generates methoxylated groups from the basic agarose structure. The main properties of these agaroses are their low melting and gelling temperatures when compared with standard agaroses.
Key features
Industry
Cloning, culture media for molecular biology, PCR and electrophoresis, proteomics.
Principles and uses
The low melting temperature allows for the recovery of undamaged nucleic acids at a temperature lower than their denaturing temperature. The low gelling temperature assures the agarose will be in a liquid state at a temperature range where In-Gel manipulations can be performed without prior extraction of the DNA from the gel slice.
Agarose LM is classified in three categories, depending on the degree of derivatization. Gelling/melting temperatures and gel strength are the most important differences.
Agarose LM is used in electrophoresis of DNA fragments (1000 bp), In-Gel enzymatic processing (digestion, ligation, PCR), preparative electrophoresis and analysis and recovery of large DNA fragments for further applications.
Some important features are:
- Lower gel strength than standard agaroses. Even so, gels can be handled easily.
- Higher clarity (gel transparency) than gels of standard agaroses.
- Great sieving capacity.
Technical specifications
| Ash | ≤ 0.4% |
|---|---|
| Clarity 1.5% (NTU) | ≤ 4 |
| Gel strength 1.5% (g/cm²) | ≥ 500 |
| Gelling temperature 1.5% (°C) | 24-28 °C |
| Melting temperature 1.5% (°C) | ≤ 65.5 °C |
| DNase/RNase activity | None detected |
| EEO | ≤ 0.12 |
| Moisture | ≤ 10% |
| Gel background | Very low |
| Color | White |
| Appearance | Fine, homogeneous powder |
| DNA resolution | Finely resolved |
| Sulphate | ≤ 0.12% |
| Storage | 2 to 25 °C |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.