Ready-to-use media
Buffered Peptone water with 0,5% Polisorbate 80
Diluent and non-selective pre-enrichment broth for the homogenization of food samples, formulated according to ISO standard.
- Cat. No.
- LC-4637
- Category
- Ready-to-use media
- Format
- 10 x 90 mL flasks
- ISO 17025 quality control
- Yes
- Storage
- 8 to 25 °C
- Minimum guaranteed shelf life
- 6 months
This formulation of Buffered Peptone Water has the advantages of the two classical diluents used for food samples: it has the property of revitalization of the peptone water and the pH change absorbing capacity of the phosphate buffer.
Key features
Applications
Diluent and enrichment with neutralizers (general use).
Industry
Food.
Presentation
10 prepared bottles, 125 mL, with 90 ± 3 mL.
Packaging
1 box with 10 bottles 125 mL. Injectable cap: plastic screw inner cap. The use of syringe needles with a diameter greater than 0.8 mm is not recommended.
Description and technique
Description
The composition of this diluent is made according to the specification of the ISO Standard 6579 for the detection of Salmonella in foods and other ISO Standards (6785, 6887 and 8261).
Polysorbate 80 was added to this culture medium to improve the emulsion of samples of foods high in fat, according to the recommendations of ISO 6887-2.
Technique
Use the medium according to intended purposes, samples and validated methods.
Presentation
| Presentation | 10 prepared bottles, 125 mL |
|---|---|
| Filling | 90 ± 3 mL |
| Packaging | 1 box with 10 bottles 125 mL. Injectable cap: plastic screw inner cap. The use of syringe needles with a diameter greater than 0.8 mm is not recommended. |
Composition
| Ingredient | g/L |
|---|---|
| Peptone | 10.0 |
| Sodium chloride | 5.00 |
| Disodium phosphate 12 H₂O | 9.00 |
| Potassium phosphate | 1.50 |
| Polysorbate 80 | 5.00 |
Quality control
Physical/chemical control
| Color | Yellow |
|---|---|
| pH | 7 ± 0.2 at 25 °C |
Microbiological control
Prepare tubes / inoculate 10³-10⁴ (productivity) / subculture after holding at 20-25 °C for 45 min to 1 h.
Microbiological control according to ISO 11133:2014/Amd 1:2018.
Aerobiosis. Incubation at 36 ± 2 °C, reading at 18 ± 2 h.
| Microorganism | Growth |
|---|---|
| Stph. aureusATCC 25923, WDCM 00034 | Good. Recovery ± 30% T0 (original enumeration) |
| Escherichia coliATCC 25922, WDCM 00013 | Good. Recovery ± 30% T0 (original enumeration) |
| Escherichia coliATCC 8739, WDCM 00012 | Good |
| Salmonella typhimuriumATCC 14028, WDCM 00031 | Good |
| Salmonella entericaATCC 13076, WDCM 00030 | Good |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ATLAS, R.M. & L.C. PARKS (1993) Handbook of Microbiological Media. CRC Press, Inc. London.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- ISO Standard 6579-1 (2017) Microbiology of food chain - Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 1: Detection of Salmonella spp.
- ISO 6785 (2001) Milk and milk products. Detection of Salmonella spp.
- ISO 6887-1 (1999) Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination. Part 1: General rules for the preparation of the initial suspension and decimal dilutions.
- ISO 6887-2 (2003) Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination. Part 2: Specific rules for the preparation of meat and meat products.
- ISO 6887-3 (2003) Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination. Part 3: Specific rules for the preparation of fish and fishery products.
- ISO 6887-4 (2003) Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination. Part 4: Specific rules for the preparation of products other than milk and milk products, meat and meat products and fish and fishery products.
- ISO/DIS 6887-5 (2009) Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination. Part 5: Specific rules for the preparation of milk and milk products.
- ISO 8261 (2001) Milk and milk products. General guidance for the preparation of test samples for microbiological examination.
- ISO 21528-1:2004 Standard. Microbiology of food and animal feeding stuffs - Horizontal methods for the detection and enumeration of Enterobacteriaceae - Part 1: Detection and enumeration by MPN technique with pre-enrichment.
- ISO/TS 22964 (2006) Milk and milk products - Detection of Enterobacter sakazakii.
- PASCUAL ANDERSON, Mª R. (1992) Microbiología Alimentaria. Díaz de Santos, S.A. Madrid.
- UNE-EN ISO 11133 (2014). Microbiología de los alimentos para consumo humano, alimentación animal y agua - Preparación, producción, conservación y ensayos de rendimiento de los medios de cultivo.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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How long does delivery take?
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