Dehydrated culture media
Buffered Saline Peptone Water
Recommended as a primary solvent for serial dilution.
- Cat. No.
- LC-1406
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Buffered Saline Peptone Water is a non-inhibitory medium, therefore allowing an easy recovery of stressed microorganisms. Buffered Saline Peptone Water is rich in nutrients and produces a high recovery of damaged cells and intensifies the growth of microorganisms. A feature common to all selective media is that sublethally injured organisms are not generally detected and therefore a recovery step must be included in examination procedures.
Key features
Applications
Diluent (general use).
Industry
General cultivation.
Preparation
9.57 g in one liter of distilled water; boil for one minute, sterilize at 121 °C for 15 minutes.
Principles and uses
Changes in pH may damage bacterial growth. Buffered Saline Peptone Water maintains a high pH via the phosphate buffer system and allows the repair of injured cells which are sensitive to low pH. Casein peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Very light beige |
| Color of the prepared medium | Colorless |
| Final pH (25 °C) | 7.0 ± 0.2 |
| Amount per litre | 9.57 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Casein peptone | 1 |
| Disodium phosphate | 0.044 |
| Sodium chloride | 8.5 |
| Sodium phosphate | 0.023 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 9.57 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize at 121 °C for 15 minutes.
Instructions for use
- Refer to the relevant references for details on test methods.
- Inoculate the tubes with the test sample.
- Incubate the tubes at 35 ± 2 °C for 18-24 hours under aerobic conditions, or as indicated in the references.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-24 h.
| Microorganism | Specification |
|---|---|
| Salmonella typhimuriumATCC 14028 | ± 30% of original count for the dilution test |
| Escherichia coliATCC 25922 | ± 30% of original count for the dilution test |
| Staphylococcus aureusATCC 25923 | ± 30% of original count for the dilution test |
Bibliography
- Standard Methods for the Examination of Water and Wastewater 15th edition, 1980.
- Juven, Cox, Bailey, Thomson, Charles and Schutze. 1984. J. Food Prot. 47:299.
- Andrews, Flowers, Silliker and Bailey. 2001. In Downes and Ito (ed.), Compendium of methods for the microbiological examination of foods, 4th ed. American Public Health Association, Washington.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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Frequently asked questions
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