Extraction and purification
CentriPure P2 Gel Filtration Columns for Protein Purification
Ready-to-use gel filtration columns for desalting and purifying proteins in 150-300 µL samples
- Cat. No.
- CP-0110-50
- Category
- Extraction and purification
- Formats
- 1 format
- Manufacturer
CentriPure P2 are pre-hydrated gel filtration columns sized for small protein samples of 150-300 µL (200 µL in the standard protocol). The spherical dextran-based composite resin retains salts, dyes, ammonium, haptens and biotin, while proteins above about 5 kDa are collected in the eluate. Separation is size-based, with minimal influence of buffer and pH, and takes about 15 minutes.
Key features
Built for small volumes
Optimised for 150 to 300 µL samples, with an elution volume of 300 to 500 µL depending on the volume loaded.
High salt removal
Removes up to 99.99% of low molecular weight impurities; the manual reports salt removal of 98.9% to 99.9%.
Reliable recovery
Recovery is normally around 85%, and up to 98% under the conditions described in the manual.
Easy handling
Pre-hydrated gravity-flow columns, equilibrated with about 5 mL of the chosen buffer.
Applications
- Desalting of small protein samples
- Buffer exchange
- Removal of free dyes after protein labelling
- Removal of unbound biotin and haptens
- Clean-up of antibodies and enzymes from small molecules
Technical details
| Matrix | Spherical dextran-based composite resin, 50-150 µm beads |
|---|---|
| Exclusion limit | About 5 kDa (proteins) |
| Sample volume | 150-300 µL (standard protocol: 200 µL) |
| Elution | 300-500 µL depending on sample volume (350 µL for 200 µL sample) |
| Recovery | Normally about 85%; up to 98% according to the manual |
| Time | About 15 minutes |
| Format | Gravity flow, pre-hydrated columns |
| Storage | +15 °C to +30 °C |
| Shipping | Ambient |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.