Extraction and purification
CentriPure P5 Gel Filtration Columns for Protein Purification
Ready-to-use gel filtration columns for desalting and purifying proteins in samples up to 500 µL
- Cat. No.
- CP-0106-50
- Category
- Extraction and purification
- Formats
- 1 format
- Manufacturer
CentriPure P5 are pre-hydrated gel filtration columns designed for desalting and purifying proteins in 450-500 µL samples. The spherical dextran-based composite resin separates by molecular size: proteins above about 5 kDa elute first, while salts, dyes, biotin, haptens and other small molecules are retained longer. Buffer and pH have only a minimal effect on the separation, and the whole run takes about 15 minutes.
Key features
Efficient small-molecule removal
Removes up to 99.99% of low molecular weight contaminants such as salts, ammonium, dyes and free biotin.
Good protein recovery
Sample recovery is normally around 85%, with a size exclusion limit of about 5 kDa.
Ready to use
Remove the caps, let the storage liquid drain by gravity and equilibrate with the same buffer used for elution.
No cold chain
Shipped at ambient temperature and stored between +15 °C and +30 °C.
Applications
- Desalting of proteins and antibodies
- Buffer exchange of protein solutions
- Removal of free fluorescent dyes after labelling
- Removal of unbound biotin and haptens after conjugation
- Clean-up of enzymes from salts and small molecules
- Protein sample preparation for downstream analysis
Technical details
| Matrix | Spherical dextran-based composite resin, 50-150 µm beads |
|---|---|
| Exclusion limit | About 5 kDa (proteins) |
| Sample volume | 500 µL (450-500 µL) |
| Elution | 1 mL buffer |
| Recovery | Normally about 85% |
| Contaminant removal | Up to 99.99% of low molecular weight molecules |
| Time | About 15 minutes |
| Format | Gravity flow, pre-hydrated columns |
| Storage | +15 °C to +30 °C |
| Shipping | Ambient |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.