Dehydrated culture media
Dextrose Broth (Glucose Broth)
For the cultivation of fastidious microorganisms and for the study of glucose fermentation.
- Cat. No.
- LC-1203
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Dextrose Broth (Glucose Broth), being highly nutritious, is used to cultivate fastidious microorganisms and specimens containing a low inoculum, as well as to detect gas formation from enteric bacilli through dextrose fermentation.
Key features
Applications
Enrichment of fastidious microorganisms.
Industry
Food.
Preparation
28 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Peptone mixture and beef extract provide nitrogen, vitamins, minerals and amino acids essential for growth. Dextrose is the fermentable carbohydrate, providing carbon and energy. Sodium chloride supplies essential electrolytes for transport and osmotic balance.
Adding 0.1-0.2% agar to Dextrose Broth helps anaerobic growth, and the dispersion of reducing substances and CO₂ formed in the environment. The low agar concentration is suitable for aerobic growth, in the clear upper zone, and for microaerophilic and anaerobic growth in the lower, flocculent agar zones.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Clear amber |
| Final pH (25 °C) | 7.3 ± 0.2 |
| Amount per litre | 28 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Dextrose | 10 |
| Beef extract | 3 |
| Peptone | 10 |
| Sodium chloride | 5 |
Preparation and use
Preparation
Suspend 28 grams of the medium in one liter of distilled water. If desired, add 0.1-0.2% of bacteriological agar. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into tubes with Durham gas collecting tubes for gas detection and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
Inoculate the medium, with or without the agar added, and incubate at 35 ± 2 °C. Read growth and gas production at 18-48 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-48 h.
| Microorganism | Specification | Characteristic reaction |
|---|---|---|
| Shigella flexneriATCC 12022 | Good growth | Gas production (-) |
| Escherichia coliATCC 25922 | Good growth | Gas production (+) |
Bibliography
- Norton, 1932. Bacteriology of pus. J. Lab. Clin. Med.
- MacFaddin J.D. 1985 Media for isolation cultivation identification maintenance of medical bacteria. Williams & Wilkins, Baltimore. MD.
What you receive
The product
Shipped under controlled temperature when the product requires it.
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