PCR and qPCR mixesDirect PCR Probe Mix SuperSens 2.0Cat. No. DB-1308Formats 2 formats
PCR and qPCR mixes
Direct PCR Gel Mix SuperSens 2.0
Ready-to-load end-point PCR mix for direct PCR without DNA extraction
- Cat. No.
- DB-1311
- Category
- PCR and qPCR mixes
- Formats
- 2 formats
- Manufacturer
- DIANA Biotechnologies
- Documents
- Instructions for use (IFU), PDFsign in to downloadInstructions for use (IFU), PDF209 KB
Description
Next generation of the Direct PCR Gel Mix with higher sensitivity and robustness. Direct PCR with no need for DNA extraction, enhanced with SuperSens technology. Contains blue and yellow tracking dyes and a density reagent to load PCR products directly on the gel and monitor migration during electrophoresis.
Key features
Unmatched sensitivity and specificity
SuperSens Taq DNA Polymerase with proprietary antibody hot-start provides the highest possible sensitivity and specificity, even in complex DNA mixtures.
Direct technology
Direct PCR without DNA extraction from human saliva and serum, yeasts, viruses and Gram-negative bacteria; Gram-positive bacteria and mammalian cell lines after a one-step pre-treatment with Lysis Buffer A.
Excellent robustness and inhibitor resistance
Ultrasensitive detection down to 1 copy of DNA; resistance to PCR inhibitors enables direct detection from biological samples.
Exceptional stability from fridge to bench
Maintains performance for at least 4 weeks at 4 °C. Probe mixes retain full sensitivity after incubation with primers and probes for up to 5 days at 25 °C.
User-friendly single-component formulation
2X ready-to-use mix: add primers, template and water.
Direct gel loading
Contains blue and yellow tracking dyes and a density reagent for direct loading of PCR products on agarose gel.
Applications
- Quantitative PCR (qPCR)
- Direct PCR from biological samples
- Multiplex qPCR
- Genotyping
- Pathogen detection
- Single-cell detection
Technical details
| Key protein | SuperSens Taq DNA Polymerase |
|---|---|
| Hot-start | Antibody-based hot-start |
| Format | 2X ready-to-use master mix, single component |
| Composition | Hot-start SuperSens Taq DNA polymerase, dNTPs, MgCl2, enhancers and stabilizers, blue and yellow tracking dyes, density reagent |
| Detection chemistry | End-point PCR with direct gel loading |
| UDG / dUTP | No |
| Sensitivity | Down to 1 copy of DNA |
| Sample input | Purified DNA or direct sample (saliva, serum, cell culture, bacteria, yeasts, viruses) |
| Kit content | 100 rxns: 1,000 µL mix (2X) + 1,000 µL PCR grade water. 1,000 rxns: 10,000 µL mix (2X) + 10,000 µL PCR grade water. |
| Storage | Store at −18 °C to −25 °C. Up to four freeze-thaw cycles tested without loss in performance. Stable for 2 years at ≤ −18 °C, up to 4 weeks at 4 °C and several days at 25 °C. |
| Bench stability | At least 4 weeks at 4 °C |
| QC assays | Functional test (PCR), E. coli gDNA absence, DNase activity absence |
| Larger sizes | 5x1,000 rxns, bulk and OEM sizes available on request |
Performance data
Manufacturer information
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Lysis buffersLysis Buffer ACat. No. DB-1281Formats 1 formatFrequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.