PCR and qPCR mixesDirect PCR Probe Mix SuperSens 2.0Cat. No. DB-1308Formats 2 formats
Lysis buffers
Lysis Buffer A
Fast genomic DNA extraction for Direct PCR mixes
- Cat. No.
- DB-1281
- Category
- Lysis buffers
- Formats
- 1 format
- Manufacturer
- DIANA Biotechnologies
- Documents
- Technical specification, PDFsign in to downloadTechnical specification, PDF164 KB
Description
Solution for fast and easy extraction of genomic DNA from cells. Supplementary reagent for Direct PCR mixes: selected viruses, bacteria or cells can be added directly into the PCR mix, while a simple one-step pre-treatment with Lysis Buffer A extends direct PCR to more complex organisms and tissues (Gram-positive bacteria such as Lactobacillus and Rhodococcus, yeasts, mammalian cell lines) and enhances sensitivity to 1 to 40 cells per reaction.
Key features
One-step pre-treatment
Add sample to the buffer, incubate, transfer lysate to the PCR mix: no column, no purification.
Enhances sensitivity of Direct PCR mixes
Brings Gram-positive bacteria and yeasts from 100 to 4,000 cells per reaction to 1 to 40 cells per reaction.
Room-temperature stable
Can be stored at room temperature (up to 25 °C) for 2 weeks.
Applications
- Direct PCR from Gram-positive bacteria
- Direct PCR from yeasts
- Direct PCR from mammalian cell lines
- Direct PCR from tissues
Technical details
| Volume | 5 mL |
|---|---|
| Use with | Direct PCR Probe, SYBR and Gel mixes SuperSens 2.0 |
| Storage | Store at 2 to 8 °C for long-term storage; up to 2 weeks at room temperature (up to 25 °C). |
Performance data
Manufacturer information
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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Tracked shipment
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PCR and qPCR mixesDirect PCR Gel Mix SuperSens 2.0Cat. No. DB-1311Formats 2 formatsFrequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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How long does delivery take?
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