Dehydrated culture media
Esculin Iron Agar
For the confirmation of Enterococcus in water by membrane filtration technique based on the esculin hydrolysis.
- Cat. No.
- LC-1574
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Esculin Iron Agar is a medium for the confirmation of enterococcal colonies on membrane filters through which water samples have been filtered and which have been incubated on an appropriate medium for enterococcus. The membrane is transferred to the Esculin Iron Agar and incubated at 41 °C for 20 minutes.
Key features
Applications
Confirmation of enterococci.
Industry
Water.
Preparation
16.5 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
The colonies turn into a black color due to the esculin hydrolysis to esculetin, which reacts with iron ions producing a blackening of the medium. Bacteriological agar is the solidifying agent.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 7.4 ± 0.2 |
| Amount per litre | 16.5 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Bacteriological agar | 15 |
| Esculin | 1 |
| Ferric ammonium citrate | 0.5 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 16.5 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121 °C for 15 minutes. Cool to 45-50 °C, mix well and dispense into plates.
Instructions for use
- Filter a measured volume of water through a membrane filter.
- Place the membrane on a plate of medium which allows the growth of enterococcus (LC-1068 TSA, LC-1109 Slanetz-Bartley Medium).
- Incubate at 37 °C for 24-48 hours.
- Transfer the membrane previously incubated, without inverting the membrane, to a plate with Esculin Iron Agar.
- Incubate at 41 °C for 20 min.
- Read the plate immediately.
Quality control
Microbiological test
Incubation conditions: confirmation (41 ± 0.5 °C / 20 min).
Inoculation conditions: membrane previously incubated.
| Microorganism | Specification | Characteristic reaction |
|---|---|---|
| Enterococcus faeciumATCC 19434 | Good growth | Black colonies |
| Enterococcus faecalisATCC 29212 | Good growth | Black colonies |
Bibliography
- Clesceri, Greenberg and Eaton (ed.). 1998. Standard methods for the examination of water and wastewater, 20th ed. American Public Health Association, Washington, D.C.
- Slanetz and Bartley. 1957. J. Bacteriol. 74:591.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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Tracked shipment
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