Dehydrated culture media
TPY Broth
Selective medium for the isolation of Bifidobacterium.
- Cat. No.
- LC-1591
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
TPY Broth is a medium recommended for Bifidobacterium.
Key features
Applications
Selective enrichment of Bifidobacterium.
Industry
Dairy products.
Preparation
36 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
The genus Bifidobacterium is the third most numerous bacterial population in the human intestine after the genera Bacteroides and Eubacterium. It is also the predominant population during childhood. Several Bifidobacterium species have been incorporated in yogurts, as they are considered to have health-promoting properties. The availability of easy and inexpensive methods for Bifidobacterium detection, identification and enumeration is consequently important in environmental and food microbiology.
Tryptone and papainic digest of soy provide nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group. Magnesium chloride, zinc sulfate, calcium chloride and ferric chloride allow the detection of small numbers or injured bifidobacteria and improve their growth. Potassium phosphates act as a buffer system. L-cysteine hydrochloride is the reducing agent.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber |
| Final pH (25 °C) | 6.5 ± 0.2 |
| Amount per litre | 36 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Dextrose | 15 |
| Calcium chloride | 0.15 |
| Dipotassium phosphate | 2 |
| Ferric chloride | 0.01 |
| Magnesium chloride anhydrous | 0.5 |
| Papainic digest of soybean | 5 |
| Tryptone | 10 |
| Yeast extract | 2.5 |
| Zinc sulfate | 0.25 |
| L-Cysteine | 0.5 |
Preparation and use
Preparation
Suspend 36 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes. The medium can be made more selective by adding 3 grams of lithium chloride, 0.1 grams of neomycin sulfate and 0.015 grams of nalidixic acid per liter of medium.
Instructions for use
Inoculate and incubate anaerobically at 35 ± 2 °C for 24 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 24 h.
Note: the microbiological test has been developed in the medium with the antibiotic solution added.
| Microorganism | Specification |
|---|---|
| Bifidobacterium breveATCC 15700 | Good growth (opaque medium) |
| Escherichia coliATCC 25922 | Inhibited growth |
| Bacillus subtilisATCC 6633 | Inhibited growth |
Bibliography
- Scardovi V. (1981): The Genus Bifidobacterium. In: The Prokaryotes Vol 2.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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Tracked shipment
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Frequently asked questions
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