Supplements and additives
Fecal Coliforms Supplement
Supplement for the isolation of fecal coliforms.
- Cat. No.
- LC-6023
- Category
- Supplements and additives
- Format
- 10 vials, each for 500 mL of medium
- Storage
- 2 to 8 °C
- Minimum guaranteed shelf life
- 15 months
- Minimum order
- 20 packs
Fecal Coliforms Broth Base (m-FC) (LC-1121) and Fecal Coliforms Agar Base (m-FC) (LC-1127) are prepared according to the formula proposed by Geldreich, Clark and Bert, and they are used for the cultivation and enumeration of fecal coliform microorganisms. These media are suitable for the membrane filtration technique at a high temperature. Many standard procedures specify the use of Fecal Coliforms Media for testing water and foods.
Key features
Applications
Growth and selective enumeration of coliforms.
Industry
Water.
Presentation
10 freeze-dried vials with 6 ± 0.1 g.
Packaging
23x60 mm glass vials, tag labelled, white plastic cap - 10 vials per box.
Description and technique
Description
Fecal coliforms are differentiated from other coliforms from environmental sources by their ability to grow at 44.5 ± 0.5 °C.
Proteose and Tryptose provide nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is the source of vitamins, particularly of the B-group, essential for bacterial growth. Lactose is the fermentable carbohydrate as a carbon and energy source. Bile salts inhibit growth of Gram-positive bacteria. Sodium chloride maintains the osmotic balance. Aniline Blue and Rosolic Acid are the differential indicators and suppress the growth of Gram-positive bacteria. Bacteriological agar is the solidifying agent.
Preparation
Aseptically reconstitute 1 vial with 5 mL of 1% solution of 0.2 N NaOH. Mix gently until complete dissolution and aseptically add to 500 mL of Fecal Coliforms Agar Base (m-FC) (LC-1127) or Fecal Coliforms Broth Base (m-FC) (LC-1121), once the medium has cooled to 45-50 °C. Mix well and distribute into sterile containers.
Instructions for use
Membrane filtration technique:
When the supplement is added to Fecal Coliforms Broth Base (m-FC) (LC-1121):
- Place the membrane filter, which the sample has been filtered through, on the upper part of the pad saturated with the medium in the Petri dish.
- Incubate the plates for 24 ± 2 hours, one batch as a control at 35 ± 2 °C, and the rest at 44.5 ± 0.5 °C.
- Observe coliforms and count the colonies.
When the supplement is added to Fecal Coliforms Agar Base (m-FC) (LC-1127):
- Place the membrane filter, which the sample has been filtered through, in the upper part of the Petri dish with the solidified agar.
- Incubate the plates for 24 ± 2 hours, one batch as control at 35 ± 2 °C, and the rest at 44.5 ± 0.5 °C.
- Observe the coliforms and count the colonies.
The differential indicator system (aniline blue and rosolic acid) gives a blue color to the colonies of fecal coliforms. The rest of enterobacteria will become red.
Presentation
| Presentation | 10 freeze-dried vials |
|---|---|
| Filling | 6 ± 0.1 g |
| Packaging | 23x60 mm glass vials, tag labelled, white plastic cap - 10 vials per box. |
Composition
| Ingredient | g/vial |
|---|---|
| Rosolic acid | 0.05 |
Reconstitute the original freeze-dried vial by adding 5 mL of 1% NaOH 0.2 N solution.
Note: each vial is sufficient to supplement 500 mL of Fecal Coliforms Agar Base (m-FC) or Fecal Coliforms Broth Base (m-FC).
Quality control
Physical/chemical control
| Color | Red - orange |
|---|
Microbiological control
Reconstitute 1 vial as indicated in Composition; shake and dissolve completely.
Add 1 vial to 500 mL of medium base. DO NOT HEAT once supplemented.
Membrane filtration / practical range 100 ± 20 CFU, min. 50 CFU (productivity) / 10⁴-10⁶ CFU (selectivity) / ≥ 10³ CFU (specificity).
Analytical methodology according to ISO 11133:2014/Amd 1:2018; A2:2020.
Aerobiosis. Incubation at 44 ± 0.5 °C. Reading at 24 ± 2 h.
Aerobiosis. Incubation at 35 ± 2 °C. Reading at 24 hours.
| Microorganism | Growth |
|---|---|
| Shigella flexneriATCC 12022, WDCM 00126 (35 °C) | Good - red colonies |
| Shigella flexneriATCC 12022, WDCM 00126 (44.5 °C) | Inhibited |
| Salmonella typhimuriumATCC 14028, WDCM 00031 (35 °C) | Good - red colonies |
| Salmonella typhimuriumATCC 14028, WDCM 00031 (44.5 °C) | Inhibited |
| Enterococcus faecalisATCC 19433, WDCM 00009 (35 °C) | Inhibited |
| Enterococcus faecalisATCC 19433, WDCM 00009 (44.5 °C) | Inhibited |
| Escherichia coliATCC 25922, WDCM 00013 (37 °C) | Good (≥ 50%) - blue colonies |
| Escherichia coliATCC 25922, WDCM 00013 (44.5 °C) | Good (≥ 50%) - blue colonies |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- Geldreich, Clark and Kabler, 1963. USPHS, HEW. Personal Communication.
- Geldreich, Clark, Huff and Bert, 1965. Journal of American Water Works Association, 57:208.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
Other products in the same category
Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.