Dehydrated culture media
Gelysate Agar (Plate Count Agar Sugar Free)
For the enumeration of microorganisms in butter and other dairy products.
- Cat. No.
- LC-1187
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
- Also available
- Ready-to-use medium, 1 format (LC-812)
Gelysate Agar (Plate Count Agar Sugar Free) is used for the enumeration of microorganisms in dairy products that are not involved in its production.
Key features
Applications
Non-selective enumeration (general use).
Industry
Dairy products.
Preparation
35 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Gelatin peptone and casein peptone provide nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride maintains the osmotic balance. Bacteriological agar is the solidifying agent. The absence of carbohydrates allows the selective cultivation of the microorganisms we are looking for.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Clear amber, slightly opalescent |
| Final pH (25 °C) | 7.5 ± 0.2 |
| Amount per litre | 35 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Bacteriological agar | 15 |
| Casein peptone | 7.5 |
| Gelatin peptone | 7.5 |
| Sodium chloride | 5 |
Preparation and use
Preparation
Suspend 35 grams of the medium in one liter of distilled water. Mix well and heat with frequent agitation. Boil for one minute until the medium dissolves completely. Sterilize in the autoclave at 121 °C for 15 minutes.
Instructions for use
Inoculate and incubate 48 hours at 35 ± 2 °C, and another 48 hours at 20 °C.
Quality control
Microbiological test
Incubation conditions: (35 ± 2 °C / 48 h) + (20 °C / 48 h).
| Microorganism | Specification |
|---|---|
| Candida albicansATCC 10231 | Good growth |
| Enterococcus faecalisATCC 11700 | Good growth |
| Bacillus cereusATCC 11778 | Good growth |
| Escherichia coliATCC 25922 | Good growth |
| Staphylococcus aureusATCC 25923 | Good growth |
| Pseudomonas aeruginosaATCC 27853 | Good growth |
Bibliography
- International Dairy Federation: Methods of sampling milk and milk products. - International Standard, FIL/IDF 50 B (1985).
- Internationaler Milchwirtschaftsverband: Zählung von Infektionskeimen in Butter. - Internationaler Standard, 153 (1991).
- Internationaler Milchwirtschaftsverband: Zählung von Infektionskeimen in Sauermilcherzeugnissen. - Internationaler Standard FIL/IDF, 153 (1991).
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.