Ready-to-use media
Giolitti-Cantoni Broth ISO
Liquid medium used for the recovery and enumeration of low numbers of coagulase-positive staphylococci in foods according to ISO, FIL-IDF and EN standards.
- Cat. No.
- LC-4228
- Category
- Ready-to-use media
- Format
- 20 tubes
- ISO 17025 quality control
- Yes
- Storage
- 8 to 14 °C
- Minimum guaranteed shelf life
- 3 months
- Standards
- ISO 6888
- Also available
- Dehydrated medium, 1 format (LC-1232)
- Also available
- Dehydrated medium, 1 format (LC-1287)
This medium for the selective enrichment of staphylococci was formulated in 1966 by Giolitti and Cantoni.
Key features
Applications
Selective enrichment and selective enumeration of Staphylococcus aureus.
Industry
Food.
Standards
ISO 6888.
Presentation
20 tubes, 16 x 113 mm, with 9 ± 0.1 mL.
Description and technique
Description
The growth of staphylococci is promoted by pyruvate, glycine and above all by a high concentration of mannitol. Addition of polysorbate 80 is necessary for the successful recovery of Staphylococcus aureus (Chopin et al., 1985). Accompanying flora are inhibited by lithium chloride and potassium tellurite.
Anaerobic growth conditions increase the selectivity of the medium. Generally, growth of staphylococci can be recognized by a blackening or black precipitates in the culture medium due to reduction of tellurite to metallic tellurium.
Technique
Refer to the standard protocol for specific products (Food and animal feeding stuffs EN-ISO 6888-3:2003; Milk and milk based products ISO 5944:2001 and FIL-IDF 60:2001).
Incubate anaerobically for 24-48 hours at 37 °C.
After 24 hours, subculture any tubes showing blackening or black precipitate by streaking onto Baird-Parker Agar. Incubate the remainder of the tubes for a further 24 hours and subculture all tubes showing growth (irrespective of blackening) to Baird-Parker Agar.
When determining the bacterial count by the MPN method, all tubes showing growth are considered as presumptive positive for staphylococci and they are confirmed only if they produce a positive result in the coagulase test.
Presentation
| Presentation | 20 tubes, 16 x 113 mm |
|---|---|
| Filling | 9 ± 0.1 mL |
| Packaging | 1 box with 20 tubes, 16 x 113 mm glass tubes, ink labelled and metal non-injectable cap. |
Composition
| Ingredient | g/L |
|---|---|
| Tryptone | 10.00 |
| Meat extract | 5.00 |
| Yeast extract | 5.00 |
| Lithium chloride | 5.00 |
| D-mannitol | 20.00 |
| Sodium chloride | 5.00 |
| Glycine | 1.20 |
| Sodium pyruvate | 3.00 |
| Polysorbate 80 | 1.00 |
| Potassium tellurite | 0.11 |
Quality control
Physical/chemical control
| Color | Yellow |
|---|---|
| pH | 6.9 ± 0.2 at 25 °C |
Microbiological control
Inoculate: practical range 100 ± 20 CFU; min. 50 CFU (productivity) / 10⁴-10⁶ (selectivity).
Microbiological control according to ISO 11133:2014/Amd 1:2018.
Aerobiosis. Incubation at 37 ± 1 °C, reading after 24/44 ± 4 h.
| Microorganism | Growth |
|---|---|
| Stph. aureusATCC 25923, WDCM 00034 | Good - black precipitate |
| Staphylococcus aureusATCC 6538, WDCM 00032 | Good - black precipitate |
| Escherichia coliATCC 8739, WDCM 00012 | Inhibited |
| Mix of the control strains | Good - black precipitate |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- CHOPIN, A. et al. (1985) ICMSF Methods Studies XV. Comparison of four media and methods for enumerating Staphylococcus aureus in powdered milk. J. Food Protect. 48:21-27.
- EN-ISO 6888-3 Standard (2003) Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of coagulase positive staphylococci (Staphylococcus aureus and other species). Part 3: Detection and MPN technique for low numbers.
- EN-ISO 6888-3/AC Standard (2005) Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of coagulase positive staphylococci (Staphylococcus aureus and other species). Part 3: Detection and MPN technique for low numbers.
- FIL-IDF (2001) Milk and milk based Products. Detection of coagulase-positive staphylococci. MPN technique. Standard 60:2001. Brussels.
- GIOLITTI, G. A. CANTONI, C. (1966) A medium for the isolation of staphylococci from foodstuffs. J. Appl. Bact. 29, 395-398.
- HARRIGAN, WF. & McCANCE, M.E. (1976) Laboratory Methods in Food and Dairy Microbiology. Academic Press. London.
- ISO 5944 Standard (2001) Milk and milk based Products. Detection of coagulase-positive staphylococci. MPN technique.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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Frequently asked questions
Can I ask for formats other than those listed?
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