Dehydrated culture media
Helicobacter Agar Modified
Selective medium for the isolation of Helicobacter pylori.
- Cat. No.
- LC-2066
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 12 months
Helicobacter Agar Modified is a selective medium for the isolation of Helicobacter pylori. Helicobacter pylori is a bacterium present in patients with chronic gastritis and gastric ulcers that was isolated the first time in 1982. More than 50% of the world's population harbor H. pylori in their upper gastrointestinal tract but most of the infected people do not show symptoms or complications. Colonization of the stomach by H. pylori can result in chronic gastritis, an inflammation of the stomach lining, at the site of infection. In the absence of treatment, H. pylori infection, once established in its gastric niche, is widely believed to persist for life. H. pylori is contagious, although the exact route of transmission is not known.
Key features
Applications
Selective isolation of Helicobacter pylori.
Preparation
42 g in one liter of distilled water; boil for one minute, do not autoclave, add 5-10% of sterile defibrinated horse blood.
Principles and uses
Beef extract, pancreatic digest of casein, peptic digest of animal tissue and yeast extract provide nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Starch in the medium acts as a growth factor, probably functioning like a colloid protector, and neutralizes toxic products that form during the development of the organisms. Bacteriological agar is the solidifying agent. The antimicrobial combination is the formulation described by Dent and McNulty, which contains combinations of vancomycin, amphotericin B, trimethoprim and cefsulodin to inhibit contaminating flora without interfering with the recovery of H. pylori.
Technical specifications
| Solubility | A slight precipitate can be found |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Toasted beige |
| Color of the prepared medium | Red |
| Final pH (25 °C) | 7.3 ± 0.2 |
| Amount per litre | 42 g |
Formula
| Ingredient | g/L |
|---|---|
| Amphotericin B | 0.005 |
| Bacteriological agar | 13.5 |
| Cefsulodin | 0.01 |
| Beef extract | 3 |
| Pancreatic digest of casein | 12 |
| Peptic digest of animal tissue | 5 |
| Sodium chloride | 5 |
| Starch | 1 |
| Trimethoprim | 0.02 |
| Vancomycin | 0.01 |
| Yeast extract | 3 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 42 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. DO NOT AUTOCLAVE. Cool to 45-50 °C and add 5-10% of sterile defibrinated horse blood. Homogenize gently and pour into Petri dishes. Be careful to avoid bubble formation when adding the blood.
Instructions for use
Inoculate and incubate at 35 ± 2 °C and observe after 18-24 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 48-72 h.
| Microorganism | Specification |
|---|---|
| Candida albicansATCC 10231 | Total inhibition |
| Bacteroides fragilisATCC 25285 | Total inhibition |
| Escherichia coliATCC 25922 | Total inhibition |
Bibliography
- Kusters JG, van Vliet AH, Kuipers EJ (July 2006). "Pathogenesis of Helicobacter pylori Infection". Clin Microbiol Rev 19 (3): 449-90. doi:10.1128/CMR.00054-05. PMC 1539101. PMID 16847081.
- Stark RM, Gerwig GJ, Pitman RS et al. (February 1999). "Biofilm formation by Helicobacter pylori". Lett Appl Microbiol 28 (2): 121-6. doi:10.1046/j.1365-2672.1999.00481.x. PMID 10063642.
- Petersen AM, Krogfelt KA (May 2003). "Helicobacter pylori: an invading microorganism? A review". FEMS Immunol. Med. Microbiol. 36 (3): 117-26. doi:10.1016/S0928-8244(03)00020-8. PMID 12738380.
- Dixon MF (February 2000). "Patterns of inflammation linked to ulcer disease". Baillieres Best Pract Res Clin Gastroenterol 14 (1): 27-40. doi:10.1053/bega.1999.0057. PMID 10749087.
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