Dehydrated culture media
Semisolid Nutrient Agar ISO
For the serological confirmation of Salmonella, detection of Salmonella species and for the detection of motility.
- Cat. No.
- LC-2046
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
- Standards
- ISO 6579
Semisolid Nutrient Agar is recommended for the serological confirmation by the antigen H of Salmonella spp. Besides, Semisolid Nutrient Agar is recommended for detection of Salmonella species on the basis of motility. Motile organisms, e.g. Salmonella, show a diffuse zone of growth spreading out from the stab inoculum line. Non-motile organisms grow only along the line.
Key features
Applications
Confirmation and detection of Salmonella.
Industry
Food, water.
Standards
ISO 6579.
Preparation
14 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Beef extract and peptones provide nitrogen, vitamins, minerals and amino acids essential for growth. The bacteriological agar is the solidifying agent which provides in this case a semisolid base suitable for the serological H confirmation.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 7.0 ± 0.2 |
| Amount per litre | 14 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Bacteriological agar | 6 |
| Beef extract | 3 |
| Peptone | 5 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 14 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121 °C for 15 minutes. Cool to 45-50 °C, mix well and dispense 15 mL into small Petri plates. Do not allow to solidify.
Instructions for use
For the serological confirmation of Salmonella:
- Inoculate the Semisolid Nutrient Agar with a pure non-agglutinable colony.
- Incubate at 37 ± 1 °C for 24 ± 3 h.
Quality control
According to ISO 11133
Incubation conditions: 34-38 °C / 24 ± 3 h.
Inoculation conditions: productivity quantitative (100 ± 20 CFU, min. 50 CFU).
| Microorganism | Specification | Characteristic reaction |
|---|---|---|
| Salmonella enteritidisATCC 13076 | Good growth | Motility (+) |
| Salmonella typhimuriumATCC 14028 | Good growth | Motility (+) |
Bibliography
- International Organization for Standardization (ISO), 1993, Draft ISO/DIS 6579
- ISO 19250. Water quality - Detection of Salmonella spp.
- Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., APHA, Washington, D.C.
- Standard Methods for the Examination of Dairy Products, 13th Ed. APHA, 1972. American Public Health Association.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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