Dehydrated culture media
Thrichomonas Broth (Kupferberg)
For selective isolation and cultivation of Trichomonas species.
- Cat. No.
- LC-2029
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Kupferberg Trichomonas Broth is used for the isolation and cultivation of Trichomonas species, and was originally formulated by Kupferberg.
Key features
Applications
Growth and selective isolation of Trichomonas.
Preparation
23.6 g in 950 mL of distilled water; boil for one minute, sterilize in autoclave at 118 °C for 15 minutes, add 50 mL of sterile calf serum or human serum.
Principles and uses
Trichomonas belongs to the flagellate group of protozoa, organisms that parasitize the intestine and urogenital systems of humans. Trichomonas hominis is a non-pathogenic protozoan whereas Trichomonas vaginalis is a frequent cause of vaginitis. Although wet mount examination of infected material is as efficient as cultures in revealing infections, current evidence suggests that cultivation methods are superior, which was earlier stated by Williams and Kean and Day. Kupferberg demonstrated the greater accuracy of the culture method, in addition to observing that the efficiency of therapy for these infections could be ascertained by using negative cultures. The culture media can be made selective for the growth of Trichomonas by the external addition of antibiotics which make the media inhibitory to the accompanying bacterial flora.
The Kupferberg Trichomonas Broth medium contains peptones, which provide the nitrogenous substances required for growth. Maltose acts as energy source. The selective agent chloramphenicol is inhibitory to accompanying Gram-positive and Gram-negative bacteria but not to Trichomonas species.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Cream |
| Color of the prepared medium | Light amber with a green halo at the top |
| Final pH (25 °C) | 6.0 ± 0.2 |
| Amount | 23.6 g in 950 mL |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Bacteriological agar | 1 |
| Casein peptone | 10 |
| Chloramphenicol | 0.1 |
| Cysteine hydrochloride | 1.5 |
| Maltose | 1 |
| Meat peptone | 10 |
| Methylene blue | 0.003 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 23.6 grams of the medium in 950 mL of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 118 °C for 15 minutes. Cool to 45-50 °C, mix well and aseptically add 50 mL of sterile calf serum or human serum.
Instructions for use
- Inoculate specimens suspected of containing Trichomonas organisms into the broth medium.
- Incubate the tubes at 30 °C for 7 days.
Quality control
The microbiological test should be carried out by the end-user laboratory.
Bibliography
- Kupferberg A. B., Johnson G. and Sprince H., 1948, Proc. Soc. Exper. Biol. Med., 67:304.
- Beal C., Goldsmith R., Kotby M., Sherid M., el-Tagi A., Farid A., Zakaria S. and Eapen J., 1992, J. Clin. Microbiol.
- Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H. (Ed.). 2003, Manual of Clinical
What you receive
The product
Shipped under controlled temperature when the product requires it.
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