Dehydrated culture media
GYC Agar
Acetic bacteria medium.
- Cat. No.
- LC-2026
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
GYC Agar, described by Swings (1992), detects the presence of acid-producing microorganisms and is regarded as "standard growth medium" for acetic acid bacteria.
Key features
Applications
Detection of mesophilic aerobes.
Industry
Food.
Preparation
85 g in one liter of distilled water; boil for one minute, avoid overheating, do not autoclave.
Principles and uses
Dextrose is the fermentable carbohydrate providing carbon and energy. Yeast extract is a source of vitamins, particularly of the B-group. Grown on this medium, Acetobacter will produce clear zones or halos around colonies because the acid being produced will neutralize the CaCO₃. Unlike the lactic acid bacteria, acetic acid bacteria are obligate aerobes and so it is necessary to use spread plates. Bacteriological agar is the solidifying agent.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Light amber, slightly opalescent |
| Final pH (25 °C) | 6.8 ± 0.2 |
| Amount per litre | 85 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Bacteriological agar | 20 |
| Calcium carbonate | 5 |
| Dextrose | 50 |
| Yeast extract | 10 |
Preparation and use
Preparation
Suspend 85 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. If desired, cool to 45-50 °C and aseptically add 70 mL of ethanol. AVOID OVERHEATING. DO NOT AUTOCLAVE. Mix well and dispense into plates.
Instructions for use
Inoculate and incubate at 28-30 °C for 3-5 days.
Quality control
Microbiological test
Incubation conditions: 28-30 °C / 3-5 days.
| Microorganism | Specification |
|---|---|
| Acetobacter acetiATCC 15973 | Good growth |
Bibliography
- Madigan M., Martinko J. (editors) (2005). Brock Biology of Microorganisms (11th ed.).
What you receive
The product
Shipped under controlled temperature when the product requires it.
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