Molecular biology media
Luria Agar with Ampicillin 100 µg/ml (Miller’s LB Agar)
For molecular studies of E. coli.
- Cat. No.
- LC-2085
- Category
- Molecular biology media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 12 months
Luria Agar with Ampicillin 100 µg/mL (Miller’s LB Agar) medium is used for the selective growth of ampicillin-resistant E. coli recombinant strains in molecular genetic studies.
Key features
Applications
Selection of Escherichia coli transformants.
Industry
Culture media for molecular biology.
Preparation
40 g in one liter of distilled water; boil for one minute, do not overheat, do not autoclave.
Principles and uses
The transformed E. coli are plated directly onto selective agar media (LB Agar containing antibiotic), where fewer transformed colonies will appear per mL plated. To select the bacteria with the plasmid, it is necessary to subculture an inoculum from LB Agar to an LB Broth with the antibiotic added.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 7.0 ± 0.2 |
| Amount per litre | 40 g |
Formula
| Ingredient | g/L |
|---|---|
| Ampicillin | 0.1 |
| Bacteriological agar | 15 |
| Sodium chloride | 10 |
| Tryptone | 10 |
| Yeast extract | 5 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 40 grams of medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. DO NOT OVERHEAT. DO NOT AUTOCLAVE. Cool to 45-50 °C, mix well and dispense into plates.
Instructions for use
- Carry out the experimental procedure according to appropriate use or purpose.
- Inoculate and incubate at 35 ± 2 °C for 18-24 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-24 h.
Inoculation conditions: productivity quantitative (100 ± 20 CFU, min. 50 CFU) / selectivity (10⁴-10⁶ CFU).
Reference medium: TSA.
| Microorganism | Specification |
|---|---|
| Escherichia coli DH5 alpha + pUC19 | Good growth > 50% |
| Escherichia coliATCC 25922 | Total inhibition |
| Escherichia coliATCC 8739 | Total inhibition |
Bibliography
- Atlas, R.M., L.C. Parks (1993) Handbook of Microbiological Media. CRC Press, Inc. London.
- The condensed protocols from molecular cloning: a laboratory manual / Joseph Sambrook, David W. Russell.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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