Molecular biology media
LB Agar (Lennox) with Ampicilin 100 µg/ml
For the cultivation of E. coli in molecular genetics studies.
- Cat. No.
- LC-2084
- Category
- Molecular biology media
- Format
- 500 g
- Storage
- 2 to 8 °C
- Minimum guaranteed shelf life
- 12 months
The LB Agar (Lennox) with Ampicillin 100 µg/mL medium is used for the selective growth of ampicillin-resistant E. coli recombinant strains in molecular genetic studies. This medium is recommended for strains that require less salt concentration.
Key features
Applications
Selection of Escherichia coli transformants.
Industry
Culture media for molecular biology.
Preparation
35 g in one liter of distilled water; boil for one minute, avoid overheating, do not autoclave.
Principles and uses
The transformed E. coli are plated directly onto selective agar media (LB Agar containing antibiotic), where fewer transformed colonies will appear per mL plated. To select the bacteria with the plasmid, it is necessary to subculture an inoculum from LB Agar to an LB Broth with the antibiotic added.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 7.0 ± 0.2 |
| Amount per litre | 35 g |
| Storage | 2 to 8 °C |
Formula
| Ingredient | g/L |
|---|---|
| Ampicillin | 0.1 |
| Bacteriological agar | 15 |
| Sodium chloride | 5 |
| Tryptone | 10 |
| Yeast extract | 5 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 35 grams of medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. AVOID OVERHEATING. DO NOT AUTOCLAVE. Cool to 45-50 °C, mix well and dispense into plates.
Instructions for use
Inoculate and incubate at a temperature of 35 ± 2 °C for 18-24 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-24 h.
| Microorganism | Specification |
|---|---|
| Escherichia coli DH5 alpha + pUC19 | Good growth |
| Escherichia coliATCC 25922 | Total inhibition |
| Escherichia coliATCC 8739 | Total inhibition |
Bibliography
- Atlas, R.M., L.C. Parks (1993) Handbook of Microbiological Media. CRC Press, Inc. London.
- The condensed protocols from molecular cloning: a laboratory manual / Joseph Sambrook, David W. Russell.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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Tracked shipment
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