Dehydrated culture media
Mannitol Salt Broth
For the isolation of pathogenic staphylococci.
- Cat. No.
- LC-1487
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Mannitol Salt Broth is a selective medium for the isolation of presumptive pathogenic staphylococci. Most of the other bacteria are inhibited by the high concentration of sodium chloride.
Key features
Applications
Detection and selective isolation of Staphylococcus.
Industry
Pharmaceutical/veterinary.
Preparation
125 g in one liter of distilled water; boil for one minute, dispense and sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Peptones provide nitrogen, vitamins, minerals and amino acids essential for growth. Mannitol is the carbohydrate energy source and phenol red is the pH indicator. Sodium chloride supplies essential electrolytes for transport and osmotic balance.
The degradation of mannitol by bacteria produces acidic products that change the color of the medium from pink to yellow. Due to its high content of sodium chloride, a heavy inoculum of the material under study can be used.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige-pink |
| Color of the prepared medium | Red |
| Final pH (25 °C) | 7.3 ± 0.2 |
| Amount per litre | 125 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Casein peptone | 12.75 |
| Dipotassium phosphate | 2.5 |
| Gelatin peptone | 4.25 |
| Mannitol | 2.5 |
| Phenol red | 0.025 |
| Sodium chloride | 100 |
| Soy peptone | 3 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 125 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
- Inoculate and incubate at 35 ± 2 °C.
- Observe after 18-48 hours and after 72 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-48 h and up to 72 h.
| Microorganism | Specification | Characteristic reaction |
|---|---|---|
| Staphylococcus epidermidisATCC 12228 | Good growth | Slight acid production (+/-) |
| Proteus vulgarisATCC 13315 | Inhibited growth | |
| Escherichia coliATCC 25922 | Inhibited growth | |
| Staphylococcus aureusATCC 25923 | Good growth | Acid production (+) |
Bibliography
- McColloch Am. J. Vet. Research, 8:173. 1947.
- Velilla, Faber, and Pelczar Am. J. Vet. Research, 8:275. 1947.
- Chapman, G.H. 1945 J. Bact. 50:201-203.
- European Pharmacopoeia. 6.5.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
Other products in the same category
Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.