Ready-to-use media
Marine Agar with Cycloheximide
Solid culture medium for heterotrophic marine bacteria.
- Cat. No.
- LC-886
- Category
- Ready-to-use media
- Format
- 20 plates, 90 mm
- ISO 17025 quality control
- Yes
- Storage
- 2 to 14 °C
- Minimum guaranteed shelf life
- 1 month
Marine Agar was formulated according to the original description of ZoBell that tries to duplicate the major mineral concentration found in sea water. Included in its composition are mineral salts, peptone and yeast extract, and growth factors necessary to sustain the growth of heterotrophic marine bacteria.
Key features
Applications
Selective enumeration of heterotrophic marine bacteria.
Presentation
20 prepared plates, 90 mm, with 21 ± 2 mL.
Packaging
1 box with 2 packs of 10 plates/pack. Single cellophane.
Description and technique
The gelling agent is agar and it is often found to be liquefied by marine bacteria. Cycloheximide is a thermostable antibiotic having a broad antibacterial spectrum which ensures the selective isolation of fungi from highly contaminated samples. Cycloheximide prevents the growth of saprophytic fungi.
Marine bacteria are thermo-sensitive and streak plates are recommended, in preference to pour plates. Incubate the samples at 20-25 °C for 48-72 h.
Presentation
| Presentation | 20 prepared plates, 90 mm |
|---|---|
| Filling | 21 ± 2 mL |
| Packaging | 1 box with 2 packs of 10 plates/pack. Single cellophane. |
Composition
| Ingredient | g/L |
|---|---|
| Meat peptone | 5.0000 |
| Yeast extract | 1.0000 |
| Iron citrate | 0.1000 |
| Sodium chloride | 19.4500 |
| Sodium sulfate | 3.2400 |
| Sodium bicarbonate | 0.1600 |
| Sodium silicate | 0.0040 |
| Sodium fluoride | 0.0024 |
| Disodium phosphate | 0.0080 |
| Calcium chloride | 1.8000 |
| Magnesium chloride | 8.8000 |
| Potassium chloride | 0.5500 |
| Potassium bromide | 0.0800 |
| Strontium chloride | 0.0340 |
| Ammonium nitrate | 0.0016 |
| Boric acid | 0.0220 |
| Agar | 15.0000 |
| Cycloheximide | 0.2000 |
Quality control
Physical/chemical control
| Color | Yellowish |
|---|---|
| pH | 7.6 ± 0.2 at 25 °C |
Microbiological control
Loop spreading.
Aerobiosis. Incubation at 20-25 °C, reading after 24/48-72 h.
| Microorganism | Growth |
|---|---|
| Vibrio alginolyticusATCC 17749 | Good |
| Vibrio parahaemolyticusATCC 17802, WDCM 00037 | Good |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- BUCK, J.D. & R.C. CLEVERDON (1960) The spread plate as a method for the enumeration of marine bacteria. Limnol. Oceanogr. 5:78-80.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- SIZEMORE, R.K. & L.H. STEVENSON (1970) Method for the isolation of proteolytic marine bacteria. Appl. Microbiol. 20:991-992.
- ZOBELL, C.E. (1941) Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J. Mar. Res. 4:42-75.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.