Ready-to-use media
Nutrient Agar EP/USP/ISO
Solid culture medium for general purpose use with less fastidious organisms according to ISO & APHA standards.
- Cat. No.
- LC-902
- Category
- Ready-to-use media
- Format
- 20 plates, 90 mm
- Storage
- 2 to 14 °C
- Minimum guaranteed shelf life
- 1 month
- Minimum order
- 15 packs
- Standards
- ISO 10273ISO 11133ISO 19250ISO 6579
- Also available
- Ready-to-use medium, 3 formats (LC-934)
- Also available
- Dehydrated medium, 2 formats (LC-1060)
ISO, APHA Nutrient Agar is a classical meat infusion medium. It is a very simple medium that can be used as a routine culture medium or as nutrient base to which growth factors can be added if necessary.
Key features
Applications
Growth and non-selective enumeration of mesophilic aerobes.
Industry
Clinical, food, water.
Standards
ISO 10273, ISO 11133, ISO 19250, ISO 6579.
Presentation
20 prepared plates, 90 mm, with 21 ± 2 mL.
Description and technique
Technique
Collect and process sample volumes according to the specifications of directives, regulations, standards or specific protocols established depending on the objectives.
Spread the plates by streaking methodology or by spiral method.
Incubate the plates upside down and under aerobic conditions at 30 ± 1 °C for 24 ± 2 h.
(Incubation times longer than those mentioned above or different incubation temperatures may be required depending on the sample, on the specifications, etc. This medium can be inoculated directly or after enrichment broth.)
After incubation, enumerate all the colonies that have appeared onto the surface of the agar.
Each laboratory must evaluate the results according to their specifications.
Calculate total microbial count per mL of sample by multiplying the average number of colonies per plate by the inverse dilution factor if a diluted sample was streaked. Report results as colony forming units (CFU) per mL or g along with incubation time and temperature.
Presentation
| Presentation | 20 prepared plates, 90 mm |
|---|---|
| Filling | 21 ± 2 mL |
| Packaging | 1 box with 2 packs of 10 plates/pack. Single cellophane. |
Composition
| Ingredient | g/L |
|---|---|
| Gelatin peptone | 5.00 |
| Meat extract | 3.00 |
| Agar | 15.00 |
Quality control
Physical/chemical control
| Color | Yellowish |
|---|---|
| pH | 6.8 ± 0.2 at 25 °C |
Microbiological control
Inoculate: practical range 100 ± 20 CFU; min. 50 CFU (productivity).
Microbiological control according to ISO 11133:2014/Amd 1:2018.
Aerobiosis. Incubation at 30 ± 1 °C during 18-24 h.
| Microorganism | Growth |
|---|---|
| Escherichia coliATCC 8739, WDCM 00012 | Good (≥ 70%) |
| Salmonella entericaATCC 13076, WDCM 00030 | Good (≥ 70%) |
| Salmonella typhimuriumATCC 14028, WDCM 00031 | Good (≥ 70%) |
| Bacillus subtilisATCC 6633, WDCM 00003 | Good (≥ 70%) |
| Yersinia enterocoliticaATCC 9610, WDCM 00038 | Good (≥ 70%) |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ATLAS, R.M., L.C. PARKS (1993) Handbook of Microbiological Media. CRC Press, Inc. London.
- EUROPEAN PHARMACOPOEIA 6.3 (2009) § 2.7.2 Microbiological Assay of Antibiotics (pg 3940). EDQM. Council of Europe. Strasbourg.
- ISO Standard 6579-1 (2017) Microbiology of food chain - Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 1: Detection of Salmonella spp.
- ISO 6785 Standard (2001). Milk and milk products - Detection of Salmonella spp.
- ISO 10273 Standard (1994) General guidance for the detection of presumptive pathogenic Yersinia enterocolitica.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.