Active ingredientMethotrexateCat. No. P-M085-001CAS 59-05-2
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Pharmacopoeial impurity · Methotrexate family
Pharmaceutical analytical standardMethotrexate EP Impurity E
Synonyms: Deoxyaminopteroic Acid, 4-[(2,4-diaminopteridin-6-yl)methyl-methylamino]benzoic acid, DAMPA, Methotrexate metabolite, NSC 131463, NSC 133723, Benzoic acid, 4-(((2,4-diamino-6-pteridinyl)methyl)methylamino)-
- Cat. No.
- P-M085-008
- CAS
- 19741-14-1
- Alternative CAS
- 70844-48-3 (hydrochloride)
- Formula
- C15H15N7O2
- Molecular weight
- 325.33 g/mol
- Category
- Pharmacopoeial impurity (EP/USP)
Family: Methotrexate
An antifolate that blocks the enzyme dihydrofolate reductase, in use since the late 1940s: born to fight childhood leukaemia, today it is used in oncology and, at low doses, for rheumatoid arthritis and psoriasis.
Did you know?In 1956 Min Chiu Li and Roy Hertz used it against choriocarcinoma, a cancer of the placenta: some patients with metastases were completely cured, the first time chemotherapy had cured a metastatic cancer.
The two levels compared
| Level 1 Mass balance | Level 2 Mass balance + qNMR | |
|---|---|---|
| Identity (IR, MS, 1H and 13C-NMR; peptides: ESI-MS, MALDI-TOF and IR) | ✓ | ✓ |
| HPLC purity (min. 95%) | ✓ | ✓ |
| Content by mass balance (min. 90%; peptides: for information only)* | ✓ | ✓ |
| Content verified by qNMR (small molecules only) | · | ✓ |
| Suitable to qualify it as an in-house primary standard (ICH Q7) | · | ✓ |
* Mass balance is performed on every molecule. The only exceptions are cases where the chemistry of the molecule prevents it, for example oils and highly volatile substances, on which some of the required measurements cannot be made: in those cases the CoA states how the content was assigned.
What you receive
The analytical standard
Shipped under controlled temperature when the molecule requires it.
Certificate of analysis (CoA)
For every lot: identity, purity and assay, with the supporting spectra and chromatograms.
Safety data sheet (SDS)
Sent with every order.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
Pharmacopoeial standards available
The official European Pharmacopoeia standards for this molecule, supplied with EDQM documentation.
- Methotrexate impurity E CRSEP Y0000664
More products in the Methotrexate family
Active ingredientMethotrexateCat. No. P-M085-001CAS 59-05-2
Active ingredientMethotrexate DisodiumCat. No. P-M085-002CAS 7413-34-5
Pharmacopoeial impurityMethotrexate EP Impurity DCat. No. P-M085-003CAS 5623-18-7
Pharmacopoeial impurityMethotrexate EP Impurity A HydrochlorideCat. No. P-M085-004CAS 73978-41-3
Pharmacopoeial impurityMethotrexate EP Impurity ACat. No. P-M085-005CAS 945-24-4
Pharmacopoeial impurityMethotrexate EP Impurity BCat. No. P-M085-006CAS 54-62-6
Pharmacopoeial impurityMethotrexate EP Impurity CCat. No. P-M085-007CAS 2410-93-7
Pharmacopoeial impurityMethotrexate EP Impurity E HemihydrochlorideCat. No. P-M085-009CAS 70844-48-3Frequently asked questions
Is mass balance always performed?
Yes, on every molecule. The only exceptions are cases where the chemistry of the molecule prevents it: for example oils and highly volatile substances, on which some measurements (such as thermogravimetry) cannot be made. In those cases the CoA states which method was used to assign the content.
Can I ask for a different quantity?
Yes. The quantities are only suggestions: choose "Other" and enter the milligrams or grams you need.
Which level should I choose?
Level 1 is enough for most routine testing. Level 2 adds an independent qNMR content value and is meant for labs that need to qualify an in-house primary standard. For peptides and similar molecules, Level 2 is not available.
When will I get the quote?
Within 24 working hours, with price, availability and delivery time.
Need a molecule you cannot find?
Custom synthesis of impurities, metabolites and labelled compounds, with the same characterisation level.