Nucleases and proteases
Proteinase K solution (20 mg/mL), PCR grade
Ready-to-use proteinase K solution at 20 mg/mL, ≥600 U/mL, PCR grade
- Cat. No.
- M3037
- Category
- Nucleases and proteases
- Formats
- 2 formats
- Manufacturer
Proteinase K from Tritirachium album already dissolved at 20 mg/mL in a glycerol storage buffer, ready to add to lysis buffers. It keeps the enzyme's broad proteolytic activity, including under denaturing conditions, and is tested for the absence of exo- and endonuclease and RNase activity. Residual DNA does not exceed 200 pg/mL, for safe use in PCR sample preparation.
Key features
Ready to use
No weighing or reconstitution: dose straight from the 20 mg/mL solution.
High activity
At least 600 U/mL, with activity rising several-fold at 50-60 °C.
Nuclease tested
No detectable degradation of lambda DNA, PhiX174 DNA or MS2 RNA in contamination tests.
Low residual DNA
Residual DNA ≤200 pg/mL, suitable for PCR sample preparation.
Applications
- DNA and RNA extraction
- Genomic DNA preparation from bacteria, tissues, cells and mouse tails
- Sample preparation for PCR
- Whole-mount in situ hybridisation
- Chromosomal DNA preparation for PFGE
- mRNA purification before cDNA synthesis
- Alkaline phosphatase denaturation after vector dephosphorylation
Technical details
| Concentration | 20 mg/mL |
|---|---|
| Activity | ≥600 U/mL; >40 U/mg protein |
| Composition | 10 mM Tris-HCl pH 7.5, 1 mM calcium acetate, 50% glycerol |
| Source | Tritirachium album (Parengyodontium album) |
| Residual DNA | ≤200 pg/mL |
| Contaminants | Exonuclease, endonuclease and RNase activity not detectable |
| Optimal conditions | pH 7.5-8.0; 50-55 °C; rapid denaturation above 65 °C |
| Working concentration | 0.05-1 mg/mL |
| CAS | 39450-01-6 |
| Storage | -20 °C |
| Shipping | On wet ice |
| Stability | 2 years at -20 °C; several months at +2/+8 °C |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.