Nucleases and proteases
Proteinase K powder, PCR grade
Broad-spectrum serine protease as lyophilised powder, PCR grade, >30 U/mg
- Cat. No.
- M3036
- Category
- Nucleases and proteases
- Formats
- 3 formats
- Manufacturer
Recombinant proteinase K from Tritirachium album, produced in Pichia pastoris and supplied as a white PCR-grade lyophilisate. This subtilisin-like protease has strong, non-specific endo- and exoproteolytic activity and stays active under denaturing conditions and over a wide pH range. No exonuclease, endonuclease or RNase activity is detectable, which makes it suitable for DNA and RNA extraction.
Key features
Active under denaturing conditions
0.2-1% SDS and 1-4 M urea stimulate its activity, an advantage in cell and tissue lysis.
Wide working range
Stable from pH 4.0 to 12.5 with an optimum at pH 7.5-8.0; activity rises several-fold from 37 °C to 50-60 °C.
PCR grade
No detectable exonuclease, endonuclease or RNase activity.
Easy to dissolve
Soluble in water at 20 mg/mL or more for stock solutions.
Applications
- Genomic DNA isolation from mammalian cells and mouse tails
- Rapid PCR template preparation from cell cultures
- Chromosomal DNA preparation for pulsed-field gel electrophoresis (PFGE)
- mRNA purification before cDNA synthesis
- Clean-up of PCR products and plasmids before cloning or in vitro transcription
- RNase and DNase inactivation in protection and run-on assays
- Removal of protein contamination
Technical details
| Source | Tritirachium album (Parengyodontium album), recombinant, expressed in Pichia pastoris |
|---|---|
| Form | White lyophilisate |
| Activity | >30 U/mg lyophilisate; >40 U/mg protein |
| Molecular weight | About 27 kDa |
| Solubility | ≥20 mg/mL in water |
| Optimal conditions | pH 7.5-8.0; 50-55 °C; rapid denaturation above 65 °C; Ca2+ protects against autolysis |
| Working concentration | 0.05-1 mg/mL |
| Inhibitors | PMSF, AEBSF; inactivated at 95 °C for at least 10 minutes |
| Contaminants | Exonuclease, endonuclease and RNase activity not detectable |
| CAS | 39450-01-6 |
| Storage | -20 °C; tolerates short periods at room temperature |
| Stability | Stock solution stable for 2 years at -20 °C |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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