Dehydrated culture media
Rappaport Vassiliadis Broth EP/USP
Enrichment medium for Salmonella.
- Cat. No.
- LC-1414
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
- Standards
- European PharmacopoeiaUSP
- Also available
- Ready-to-use medium, 2 formats (LC-4016)
Rappaport Vassiliadis Broth is recommended as the selective enrichment medium when isolating Salmonella species from food and environmental specimens and is recommended by the European Pharmacopoeia in paragraph 2.6.13 "Microbiological examination of non-sterile products: test for specified microorganisms" for the test of Salmonella in products.
Key features
Applications
Selective enrichment of Salmonella.
Industry
Final product quality control, food, pharmaceutical/veterinary, water.
Standards
European Pharmacopoeia, USP.
Preparation
27.11 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 115 °C for 15 minutes.
Principles and uses
Soy peptone provides nitrogen, vitamins and amino acids, essential nutrients for growth. Potassium phosphates balance the low pH of the medium, combined with the presence of magnesium chloride to raise the osmotic pressure, and malachite green to inhibit other organisms.
This medium has been found to be superior to other Salmonella selective enrichment media, especially when small inocula and a pre-enrichment broth are used.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Blue greenish |
| Color of the prepared medium | Blue |
| Final pH (25 °C) | 5.2 ± 0.2 |
| Amount per litre | 27.11 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Dipotassium phosphate | 0.4 |
| Magnesium chloride anhydrous | 13.58 |
| Malachite green | 0.036 |
| Monopotassium phosphate | 0.6 |
| Sodium chloride | 8 |
| Soy peptone | 4.5 |
Preparation and use
Preparation
Suspend 27.11 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 115 °C for 15 minutes.
Instructions for use
For the test for specified microorganisms (Salmonella) according to the European Pharmacopoeia:
- After pre-enrichment with Trypticasein Soy Broth (TSB) (LC-1224), transfer 0.1 mL to 10 mL of Rappaport Vassiliadis Broth.
- Incubate at 30-35 °C for 18-24 hours.
- Subculture on plates of XLD Agar (LC-1080) and incubate at 30-35 °C for 18-48 hours.
- The possible presence of Salmonella is indicated by the growth of well-developed, red colonies with or without black centers. These results can be confirmed with identification tests.
- The product complies with the test if colonies of the types described are not present, or if the confirmatory identification tests are negative.
Quality control
According to European Pharmacopoeia
Incubation conditions: 30-35 °C / 18-24 h.
Inoculation conditions: productivity (≤ 100 CFU) / inhibitory (≥ 100 CFU).
| Microorganism | Specification |
|---|---|
| Salmonella typhimuriumATCC 14028 | Good growth, turbidity |
| Staphylococcus aureusATCC 6538 | Inhibited |
Bibliography
- European Pharmacopoeia 9.0
What you receive
The product
Shipped under controlled temperature when the product requires it.
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Frequently asked questions
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