Nucleases and proteases
Ribonuclease A (RNase A), DNase-free
DNase-free endoribonuclease from bovine pancreas, ≥50 U/mg, ready for DNA purification
- Cat. No.
- S5218
- Category
- Nucleases and proteases
- Formats
- 2 formats
- Manufacturer
RNase A from BSE-free bovine pancreas, supplied as a salt-free lyophilised powder free of DNase activity. It cleaves single-stranded RNA on the 3' side of pyrimidines; at low ionic strength (100 mM NaCl) it also degrades double-stranded RNA and RNA:DNA hybrids, while above 300 mM NaCl it acts only on single strands. Because DNases and proteases are not detectable, it can be added directly to DNA purification procedures.
Key features
DNase-free
DNase and protease activity not detectable: use directly, with no heat pretreatment.
Tunable specificity
Activity on double-stranded RNA and RNA:DNA hybrids depends on salt concentration.
Wide working range
Active from pH 6 to 10 (optimum 7.6) and from 15 to 70 °C (optimum 60 °C).
Controlled origin
Made from BSE-free bovine material.
Applications
- RNA removal in plasmid preparations and DNA purification
- RNase protection assays
- Removal of non-specifically bound RNA
- RNA sequence analysis
- Hydrolysis of RNA in protein samples
- Blunt-ending of double-stranded cDNA
Technical details
| Source | Bovine pancreas, BSE-free |
|---|---|
| Form | Salt-free lyophilised powder |
| Activity | ≥50 U/mg (Kunitz) |
| Molecular weight | About 13.7 kDa; pI 9.6 |
| Contaminants | DNases and proteases not detectable |
| Optimal conditions | pH 7.6 (active at pH 6-10); 60 °C (active at 15-70 °C) |
| Working concentration | 10 µg/mL for plasmid preparations (1 hour at room temperature); 100 ng/mL for double-stranded cDNA |
| Stock solutions | 1-10 mg/mL in 10 mM Tris-HCl pH 7.5 with 15 mM NaCl, or in TE |
| Inhibitors | RNase inhibitor, DEPC, guanidinium salts, beta-mercaptoethanol, heavy metals, vanadyl complexes |
| CAS | 9001-99-4 |
| Storage | +2/+8 °C, kept dry |
| Shipping | Ambient temperature |
| Stability | Lyophilised: several years at +2/+8 °C; in solution: several years at -20 °C, a few weeks at +2/+8 °C |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.