Nucleases and proteases
Ribonuclease A (RNase A), bovine pancreas
High-activity endoribonuclease from bovine pancreas, ≥70 U/mg, powder
- Cat. No.
- S5231
- Category
- Nucleases and proteases
- Formats
- 1 format
- Manufacturer
RNase A from BSE-free bovine pancreas with a minimum activity of 70 Kunitz units per mg. It cleaves single-stranded RNA on the 3' side of pyrimidines and, at low salt concentration, also double-stranded RNA and RNA:DNA hybrids. It is not certified DNase-free: before use in DNA procedures a heat treatment to inactivate DNases is recommended.
Key features
High specific activity
At least 70 U/mg (Kunitz).
Wide working range
Active from 15 to 70 °C (optimum 60 °C) and from pH 6 to 10 (optimum 7.6).
Simple DNase inactivation
Dissolve at 10 mg/mL in sodium acetate pH 5.2, heat at 100 °C for 15 minutes and readjust the pH with 1 M Tris-HCl pH 7.4.
Controlled origin
Made from BSE-free bovine material.
Applications
- RNA removal in plasmid preparations and DNA purification, after DNase inactivation
- RNase protection assays
- Removal of non-specifically bound RNA
- RNA sequence analysis
- Hydrolysis of RNA in protein samples
- Blunt-ending of double-stranded cDNA
Technical details
| Source | Bovine pancreas, BSE-free |
|---|---|
| Form | Powder |
| Activity | ≥70 U/mg (Kunitz) |
| Molecular weight | About 13.7 kDa |
| DNase | Not certified DNase-free; heat inactivation recommended before use |
| Optimal conditions | pH 7.6 (active at pH 6-10); 60 °C (active at 15-70 °C) |
| Working concentration | 10 µg/mL for plasmid preparations (1 hour at room temperature); 100 ng/mL for double-stranded cDNA |
| Stock solutions | 1-10 mg/mL in 10 mM Tris-HCl pH 7.5 with 15 mM NaCl, or in TE |
| Inhibitors | RNase inhibitor, DEPC, 4 M guanidinium thiocyanate, beta-mercaptoethanol, heavy metals, vanadyl complexes |
| CAS | 9001-99-4 |
| Storage | +2/+8 °C |
| Shipping | Ambient temperature |
| Stability | Dry: several years at +2/+8 °C; in solution: several years at -20 °C, a few weeks at +2/+8 °C |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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Tracked shipment
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Frequently asked questions
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