Dehydrated culture media
Sabouraud Dextrose Agar + Cycloheximide
For the selective cultivation of yeasts and molds.
- Cat. No.
- LC-1088
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Sabouraud Cycloheximide Dextrose Agar is a selective medium that can be used for cultivating yeasts, molds and aciduric microorganisms. It is also used for cultivating pathogenic fungi, particularly those associated with skin infections. This medium is at the same time used for determining the microbial and fungal content of cosmetics and for the mycological evaluation of food.
Key features
Applications
Growth of pathogenic fungi and yeasts.
Industry
Antimicrobial susceptibility testing, cosmetics, food.
Preparation
65.4 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 118-121 °C for 15 minutes.
Principles and uses
Dextrose is the fermentable carbohydrate that provides carbon and energy. Peptone mixture is a source of nitrogen, vitamins and amino acids. Bacteriological agar is the solidifying agent. The high dextrose concentration and acidic pH make this medium selective for fungi.
This medium is a modification of the Dextrose Agar described by Sabouraud, with the addition of cycloheximide. Cycloheximide is an antibiotic which inhibits saprophytic fungi but allows the growth of pathogenic fungi: Cryptococcus neoformans, Aspergillus fumigatus and some species of Candida (albicans, krusei).
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 5.6 ± 0.2 |
| Amount per litre | 65.4 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Dextrose | 40 |
| Bacteriological agar | 15 |
| Cycloheximide | 0.4 |
| Peptone mixture | 10 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 65.4 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Distribute and sterilize in autoclave at 118-121 °C for 15 minutes. AVOID OVERHEATING as it facilitates the hydrolysis of the components and the medium remains soft.
Instructions for use
- For cosmetic, food or environmental monitoring samples, follow appropriate standard methods for details on sample collection and preparation according to sample type and geographic location.
- Incubate at 30 °C for 3-7 days.
Quality control
Microbiological test
Incubation conditions: 30 °C / 3-7 days.
| Microorganism | Specification |
|---|---|
| Aspergillus brasiliensisATCC 16404 | Light/inhibited growth |
| Candida albicansATCC 2091 | Good growth |
| Trichophyton mentagrophytesATCC 24957 | Good growth |
| Escherichia coliATCC 25922 | Good/moderate growth |
Bibliography
- M.R. Pascual Anderson (1982) Técnicas para Análisis Microbiológico de Alimentos y Bebidas.
- Sabouraud R. 1892. Ann. Dermatol. Syphilol. 3:1061.
- Jarett, L., and A.C. Sonnenwirth (ed) 1980. Gradwohl's clinical laboratory methods and diagnosis, 8th ed. CV Mosby.
- Curry, A. S., J. G. Graf, and G. N. McEwen, Jr. (ed) 1993. CTFA Microbiology Guidelines. The Cosmetic, Toiletry, and Fragrance Association, Washington, D.C.
What you receive
The product
Shipped under controlled temperature when the product requires it.
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