Dehydrated culture media
Sabouraud Fluid Medium
For the cultivation of yeasts and molds.
- Cat. No.
- LC-1506
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Sabouraud Fluid Medium is employed in sterility test procedures for determining the presence of molds, yeasts and aciduric microorganisms. Sabouraud Fluid Medium is also used in the sterility tests of pharmaceutical products, especially parenterals, such as antisera, antibiotic preparations, venipuncture equipment, and saline and glucose solutions. It is recommended in the USP for the determination of the fungistatic activity of pharmaceutical and cosmetic products to prevent false sterility tests.
Key features
Applications
Selective enrichment of yeasts and molds.
Industry
Pharmaceutical/veterinary.
Preparation
30 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes, do not overheat.
Principles and uses
Dextrose is the fermentable carbohydrate providing carbon and energy. Casein and meat peptones provide nitrogen, vitamins, minerals and amino acids essential for growth. The acidic pH of the final medium is inhibitory to a large number of bacteria and makes the medium particularly well-suited for cultivating fungi and acidophilic microorganisms.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber |
| Final pH (25 °C) | 5.7 ± 0.2 |
| Amount per litre | 30 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Dextrose | 20 |
| Casein peptone | 5 |
| Meat peptone | 5 |
Preparation and use
Preparation
Suspend 30 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes. DO NOT OVERHEAT, since the medium contains high levels of carbohydrates that can caramelize (darken) and lose effectiveness.
Instructions for use
- Take the inoculum with a sterile loop.
- Submerge the loop into the medium and shake gently.
- Incubate at 30 ± 2 °C for 18-48 hours.
Quality control
Microbiological test
Incubation conditions: 30 ± 2 °C / 18-48 h.
| Microorganism | Specification |
|---|---|
| Candida albicansATCC 10231 | Good growth |
| Aspergillus brasiliensisATCC 16404 | Good growth |
| Escherichia coliATCC 25922 | Partially inhibited growth |
| Lactobacillus rhamnosusATCC 9595 | Good growth |
| Saccharomyces cerevisiaeATCC 9763 | Good growth |
Bibliography
- Grove and Randall, Assay Methods of Antibiotics. Medical Encyclopedia. Inc. New York, 1958.
- Davidson, A.M. and E.S. Dowding, and A.H.R. Buller. 1932. Hyphal fusions in dermatophytes. Can. J. Res. 6:1.
- United States Pharmacopoeial Convention. 1995. The United States Pharmacopoeia, 23rd ed. The United States Pharmacopoeial Convention, Rockville, M.D.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
Other products in the same category
Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.